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- 2017 (3) (entfernen)
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- Bisphenol A (2)
- ELISA (2)
- BPA (1)
- DCF (1)
- Diclofenac (1)
- FPIA (1)
- GNP (1)
- Immunoassay (1)
- LFIA (1)
- Passive absorption (1)
Pharmacologically active compounds are often detected in wastewater and surface waters. The nonsteroidal anti-inflammatory drug diclofenac was included in the watch list of substances in the EU that requires their environmental monitoring in the member states. DCF may harmfully influence the ecosystem and the natural organization already at concentrations of ≤1 μg/l. The fast and easy quantification of DCF is becoming a subject of global importance. Fluorescence polarization immunoassay (FPIA) is a homogeneous mix-and-read method, which does not require the immobilization of reagents, they can be performed in one phase, making it possible to analyze wastewater without any complicated pre-treatment, performed within 20–30 min.
Bisphenol A (BPA) is a common industrial chemical that is widely used as a monomer or additive in the production of polymer materials. This substance causes impairment of the childbearing function, acceleration of sexual aging, negatively impacts the brain, and also contributes to the development of certain oncological diseases, as well as increasing the risk of a fetus developing the Down syndrome. But the biggest downside of bisphenol is the fact that it accumulates in the system during its use, as well as after. Many methods have been proposed for the determination of BPA, in particular, gas and liquid chromatography. The main disadvantages of such approaches are the high cost of the equipment, the significant duration of an analysis, and difficulties in screening a large number of samples. Immunoanalytical techniques attributes are high throughput, high specificity and sensitivity, as well as low cost and simplicity. Particularly interesting as a quick test for screenings is the lateral flow immunoassay (LFIA), a format widely in use for the detection of pregnancy. LFIA for BPA have been described before, but lack sensitivity.
In this work, focuses on the conjugation (by passive adsorption) efficiency of the different monoclonal antibodies with 40 nm gold nanoparticles. The effect of factors such as pH value and concentration of antibody has been quantificationally discussed using spectra methods after adding 10% NaCl which induced gold nanoparticle aggregation. Consequently, 40 nm gold nanoparticles should be labeled with antibody under optimal pH value and optimal concentrations of antibody. It will be helpful for the application of antibody-labeled gold nanoparticles for the determination of bisphenol A in aqueous solutions (water from different sources, leachates and extracts).
Bisphenol A (BPA) is a common industrial chemical that is widely used as a monomer or additive in the production of polycarbonate plastics and other polymeric materials. The primary way of exposure to BPA for most people is assumed to be a direct con-tamination of food and water through use of containers, bottles, and other plastic stor-age. BPA has been identified as an important endocrine disrupting compound (EDC). It can interfere with hormonal activities like biosynthesis, metabolism and elimination of natural blood-borne hormones. Moreover, BPA can originate environmental problems. A spectrum of methods have been developed with the aim of determining BPA in water. Immunoassays appear to comply with sensitivity, rapidity, and low cost requirements for effective monitoring.
In this work a comparative analysis was conducted of the effect of temperature and the choice of buffers, as well as the substrate at various stages of an ELISA (enzyme-linked immunosorbent assay). The method has been optimized and has shown good repeatability and sensitivity. The results obtained from ELISA will be used as the basis for the development of a Lateral Flow ImmunoAssay (LFIA).