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Autor

  • Vandrich, Jasmina (5)
  • Hertlein, Aljona (1)

Erscheinungsjahr

  • 2018 (2)
  • 2017 (1)
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  • Halomonas CRISPR Ectoine (3)
  • CRISPR Genetic Engineering (1)
  • CRISPR Genetic Engineering Ectoine (1)

Organisationseinheit der BAM

  • 4 Material und Umwelt (5)
  • 4.1 Biologische Materialschädigung und Referenzorganismen (5)

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CRISPR/CAS9 Revolution in genetic engineering? (2015)
Vandrich, Jasmina ; Hertlein, Aljona
Just in 2005 the CRISPR structures were discovered to function as an adaptive immune system in prokaryotes (Koonin et al., 2005). Today, the CRISPR/Cas9 system is the most promising tool for future genetic engineering in all organisms.
Maximizing ectoine production (2016)
Vandrich, Jasmina
Genetic engineering with CRISPR/Cas9 in Halomonas elongata
Tampering with ectoine production and excretion in Halomonas elongata using CRISPRi and gene knock-outs (2018)
Vandrich, Jasmina
The halophilic bacterium Halomonas elongata can tolerate salt concentrations above 10% NaCl and uses the accumulation of the compatible solute ectoine as a major osmoregulatory mechanism. Ectoine can be accumalted inside the cell through import from the medium or de novo synthesis and establishes an osmotic equilibrium with the surrounding1. Ectoine also protects proteins from the effects of freezing, drying and high temperatures4 and DNA from ionizing radiation2. These features make ectoine a valuable compound for cosmetics and medical devices. H. elongata was originally isolated from a solar salt facility, where it thrives under high salt concentrations. It was found that marine prokaryotes, which are exposed to high oxidative stress in their environment, vary glycolytic strategies5. A variation in the use of the glucose metabolic pathways is also assumed for H. elongata.
Establishing CRISPRi in Halomonas elongata to uncover metabolic pathways (2018)
Vandrich, Jasmina
The halophilic bacterium Halomonas elongata can tolerate salt concentrations above 10% NaCl and uses the accumulation of the compatible solute ectoine as a major osmoregulatory mechanism. Ectoine can be accumalted inside the cell through import from the medium or de novo synthesis and establishes an osmotic equilibrium with the surrounding1. Ectoine also protects proteins from the effects of freezing, drying and high temperatures4 and DNA from ionizing radiation2. These features make ectoine a valuable compound for cosmetics and medical devices. H. elongata was originally isolated from a solar salt facility, where it thrives under high salt concentrations. It was found that marine prokaryotes, which are exposed to high oxidative stress in their environment, vary glycolytic strategies5. A variation in the use of the glucose metabolic pathways is also assumed for H. elongata.
CRISPRi for optimized ectoine production in Halomonas elongata (2017)
Vandrich, Jasmina
The CRISPR-Cas9 system revolutionized genetic engineering approaches with its simple design and high accuracy. The system comprises only two components – a synthetic guide RNA and the Cas9 protein. Our aim is to use this system to study factors influencing ectoine production. Here, we use the CRISPRinterference variant to downregulate the expression of the ectoine synthesis gene ectA.
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