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Schlagworte
The CRISPR-Cas9 system revolutionized genetic engineering approaches with its simple design and high accuracy. The system comprises only two components – a synthetic guide RNA and the Cas9 protein. Our aim is to use this system to study factors influencing ectoine production.
Here, we use the CRISPRinterference variant to downregulate the expression of the ectoine synthesis gene ectA.