Filtern
Erscheinungsjahr
Dokumenttyp
- Zeitschriftenartikel (117)
- Vortrag (49)
- Posterpräsentation (16)
- Beitrag zu einem Tagungsband (3)
- Buchkapitel (2)
- Sonstiges (1)
Schlagworte
- Immunoassay (48)
- ELISA (36)
- Antikörper (12)
- Biosensoren (12)
- Carbamazepine (12)
- Abwasser (10)
- Antibody (10)
- Biomarkers (9)
- Biosensor (9)
- LC-MS/MS (9)
- Pharmaceuticals (9)
- Caffeine (8)
- Koffein (8)
- Wastewater (8)
- Bisphenol A (6)
- Bivalves (6)
- Schnelltest (6)
- Carbamazepin (5)
- Characterization (5)
- Ethinylestradiol (5)
- Oxidative stress (5)
- Pharmaceutical drugs (5)
- Cetirizine (4)
- Diclofenac (4)
- Fenton oxidation (4)
- SARS-CoV-2 (4)
- Surface water (4)
- Vor-Ort-Analytik (4)
- Amoxicillin (3)
- Anthropogenic markers (3)
- Atrazine (3)
- Bead-based assay (3)
- Biomarker (3)
- Bisphenol A degradation (3)
- Clams (3)
- Cocaine (3)
- EE2 (3)
- Electrochemical detection (3)
- Elektrochemisch (3)
- Endokrine Disruptoren (3)
- Environment (3)
- Invertebrates (3)
- Microfluidic (3)
- Nanocatalysts (3)
- Oberflächenwasser (3)
- Ocean acidification (3)
- Pharmazeutika (3)
- Photodegradation (3)
- Schnelltests (3)
- Soil (3)
- Sorption (3)
- Toxikologie (3)
- ZIF (3)
- Amperometry (2)
- Antibiotika (2)
- Arzneimittel (2)
- Automation (2)
- CZE (2)
- Cetirizin (2)
- Competitive assay (2)
- Compost (2)
- Degradation (2)
- Electrochemical (2)
- Electrochemistry (2)
- Endocrine disruptor (2)
- Energy reserves (2)
- Enzyme-Linked Immunosorbent Assay (2)
- Enzyme-linked immunosorbent assay (2)
- Estradiol (2)
- FPIA (2)
- Fenton (2)
- Flow cytometry (2)
- Flow-cytometry (2)
- Fluorescence (2)
- Fluorescence polarization immunoassay (2)
- Hormone (2)
- Immunization monitoring (2)
- Katalysator (2)
- Konjugate (2)
- LC-ELISA (2)
- LFIA (2)
- Lab-on-chip (2)
- MOF (2)
- Magnetic beads (2)
- Magnetic nanocatalyst (2)
- Magnetpartikel (2)
- Mass spectrometry (2)
- Matrix effects (2)
- Micropollutants (2)
- Mikrofluidik (2)
- Monoclonal antibodies (2)
- Monoklonal (2)
- Muscheln (2)
- Oberflächengewässer (2)
- Oxidative Stress (2)
- Oxidative stress biomarkers (2)
- Photooxidation (2)
- Porphyrin (2)
- Schadstoffe (2)
- Sensor (2)
- Sewage sludge (2)
- Speichel (2)
- Standard measurement procedure (2)
- Sulfamethoxazole (2)
- Therapeutische Antikörper (2)
- Umweltschadstoffe (2)
- Validierung (2)
- Wasser (2)
- Wastewater treatment plants (2)
- Wastewater-based epidemiology (2)
- Water treatment (2)
- X-Ray diffraction (2)
- 13C-NMR (1)
- 17 beta-Estradiol (1)
- 17alpha-Ethinylestradiol (1)
- 17alpha-ethinylestradiol (1)
- 17beta-Estradiol (1)
- 17beta-estradiol (1)
- 3R concept (1)
- 3ß-hydroxy-5ß-cholanic acid (1)
- Abbau (1)
- Acoustic cavitation (1)
- Acquired immunodeficiency syndrome (1)
- Acute toxicity (1)
- Adsorption-desorption (1)
- Advanced oxidation processes (1)
- Aflatoxin (1)
- Amino acid analysis (1)
- Animal welfare (1)
- Anthropogene Marker (1)
- Antibodies (1)
- Antibody production for hydrophobic molecules (1)
- Antibody quantification (1)
- Antiepileptika (1)
- Antiepileptikum (1)
- Antifouling surface (1)
- Antigen testing (1)
- Antihistamines (1)
- Antihistaminika (1)
- Antikörperentwicklung (1)
- Antikörperkonjugate (1)
- Array (1)
- Arzneistoffe (1)
- Assay (1)
- Assay format (1)
- Assay optimization (1)
- Azithromycin (1)
- BSA (1)
- Bakterien (1)
- Bead injection spectroscopy (1)
- Bead injections (1)
- Beschichtung (1)
- Bile acids (1)
- Biochemische Parameter (1)
- Biochemische Sensoren (1)
- Biokonjugate (1)
- Biokonjugation (1)
- Biosensors (1)
- Bioseparation (1)
- Blasenkrebs (1)
- Brunauer-Emmett-Teller (1)
- CCQM-K126 (1)
- CD104 (1)
- CD4 expression level (1)
- CD4+ cell counting (1)
- CLSM (1)
- Calibration (1)
- Capillary electrophoresis (1)
- Catalytic wet peroxide oxidation (1)
- Cetrizin (1)
- Characterization of catalysts (1)
- Chemie (1)
- Chemometrics (1)
- Chemometrie (1)
- Chlorine (1)
- Clarithromycin (1)
- Climate change (1)
- Conjugates (1)
- Coproantibodies (1)
- Core−shell particles (1)
- Cowpea mosaic virus (1)
- Crystal structure (1)
- Cyclic voltammetry (1)
- Cyclization (1)
- Cytochrome P450 CYP1A2 (1)
- DEHT (1)
- DINCH (1)
- DLLME (1)
- DNA interstrand cross-link (ICL) (1)
- Decaffeinated coffee (1)
- Deconvolution (1)
- Design of experiments (1)
- Diclophenac (1)
- Digoxigenin (1)
- Diopatra neapolitana (1)
- Direct (1)
- Dirithromycin (1)
- Drogenanalytik (1)
- Drugs on bills (1)
- Durchflußzytometrie (1)
- E. coli (1)
- EDTA (1)
- ELISA immunoassay (1)
- ESR (1)
- Eintrag (1)
- Electrochemical immunosensor (1)
- Elektrochemie (1)
- Elektrochemischer Sensor (1)
- Emerging Pollutants (1)
- Endocrine disrupting compounds (1)
- Endocrine disruption (1)
- Endokriner Disruptor (1)
- Environmental monitoring (1)
- Environmentally relevant concentrations (1)
- Enyzme (1)
- Enzyme immunoassay (1)
- Enzyme immunoassays (1)
- Enzyme substrate (1)
- Equivalent fluorescein fluorophore (EFF) (1)
- Ergot alkaloids (1)
- Ersatzstoffe (1)
- Erythromycin (1)
- Estrogen (1)
- Estrogens (1)
- Estrone (1)
- Euro banknotes (1)
- Explosive (1)
- FACS (1)
- FITC (1)
- FT-IR (1)
- Farmyard manure (1)
- Fe-based highly active ionic liquids (1)
- Fecal marker (1)
- Feces (1)
- Ferrous oxalate (1)
- Festphasenextraktion (1)
- Few-layered titanium carbide nanosheets (1)
- Five iron oxalate core–shell magnetite nanoparticles catalysts are evaluated as magnetic heterogeneous (1)
- Flow injection analysis (1)
- Fluorescence Polarization (1)
- Fluoreszenz (1)
- Food analysis (1)
- Fow injection analysis (1)
- Freisetzung (1)
- Furanmodified DNA duplexes (1)
- Gastric cancer (1)
- Geschichte (1)
- Getränke (1)
- Gewässer (1)
- HPLC-MS (1)
- HPLC–MS (1)
- Hapten (1)
- Hapten design (1)
- Hapten immunoassay (1)
- Hapten synthesis (1)
- Heterogeneous photocatalysis (1)
- Hochauflösende Massenspektrometrie (1)
- Homogeneous immunoassay (1)
- Horseradish peroxidase (1)
- Human immunodeficiency virus-1 (1)
- Human serum (1)
- Hybridoma (1)
- Hybridoma cells (1)
- Hydrodynamic cavitation (1)
- Hydrolyseprodukte (1)
- Hydrolysis (1)
- Hydrolysis products (1)
- Hydroxycarbamazepine (1)
- ICP-MS (1)
- IgG (1)
- Immunassay (1)
- Immunization (1)
- Immunoarray (1)
- Immunoassays (1)
- Impedanz (1)
- Indirect (1)
- Invertebraten (1)
- Kinetics (1)
- Klimaerwärmung (1)
- Kläranlage (1)
- Kokain (1)
- Kopplungsmethoden (1)
- Krebstherapie (1)
- Kreuzreaktivität (1)
- Kristallstruktur (1)
- Kristallstrukturen (1)
- LCMS/MS (1)
- LCMSMS (1)
- LED-induzierte Fluoreszenz (1)
- Lab-on-valve (1)
- Label (1)
- Label-free detection (1)
- Labels (1)
- Lateral Flow Assay (1)
- Layer-by-Layer (1)
- Lebensmittelanalytik (1)
- Liver function tests (1)
- Long-term exposure (1)
- Long-term exposures (1)
- Lyophilized cells (1)
- MALDI-TOF-MS (1)
- MALDI-ToF (1)
- Macrolide antibiotics (1)
- Magnetic coreshell nanocatalysts (1)
- Magnetic particles (1)
- Magnetische Nanopartikel (1)
- Makrolide (1)
- Markierung (1)
- Matrixeffekte (1)
- Measurement of uncertainty (1)
- Measurement uncertainty (1)
- Meer (1)
- Mesofluidics (1)
- Mesoporöses Silica (1)
- Metabolite (1)
- Metal mobility (1)
- Metalle (1)
- Microarray (1)
- Microfluidics (1)
- Microparticles (1)
- Microporous (1)
- Mitigation (1)
- Mix-and-read assay (1)
- Mixed surface (1)
- Molecular Organic Frameworks (1)
- Monitoring (1)
- Monoklonale Antikörper (1)
- Monosaccharide (1)
- Multiplex (1)
- Multivariate data analysis (1)
- Mycotoxins (1)
- Nano (1)
- Nanogold (1)
- Nanoparticle (1)
- Nanoparticles (1)
- Nanopartikel (1)
- Nanoteilchen (1)
- Ochratoxin A (1)
- Oleandomycin (1)
- Oligo (1)
- On-site measurement (1)
- Organic amendments (1)
- Organic matter charcterization (1)
- Organic pollutants (1)
- Paper based devices (1)
- Paramagnetic beads (1)
- Partial least squares regression (1)
- Particles (1)
- Partikel (1)
- Patent (1)
- Peak identification (1)
- Peptide analysis (1)
- Pesticide parathion-methyl (1)
- Pharmaceutical (1)
- Phenols (1)
- Photo-Fenton oxidation (1)
- Photodynamische Inaktivierung (1)
- Photodynamische Therapie (1)
- Photoimmunoconjugate (1)
- Photoimmunotherapy (1)
- Photokatalyse (1)
- Photosensibilisator (1)
- Phthalate (1)
- Phthalate substitutes (1)
- Phthalocyanin (1)
- Phthalocyanine (1)
- Phthalocyanines (1)
- Point-of-Care (1)
- Pollution (1)
- Polyclonal antibodies (1)
- Polyelektrolyt (1)
- Polymere (1)
- Porphyrine (1)
- Porphyrins (1)
- Portable Analytik (1)
- Portugal (1)
- Probenvorbereitung (1)
- Proteine microarray (1)
- Quality assurance criteria (1)
- Quantification (1)
- Quantitative flow cytometry (1)
- Reactive azo dye degradation (1)
- Recombinant (1)
- Reference cell material (1)
- Reference material (1)
- Regenerative capacity (1)
- Relative concentration measurement (1)
- Removal (1)
- Rinderserumalbumin (1)
- Round robin test (1)
- Roxithromycin (1)
- SAFIA (1)
- SARS-CoV-2 nucleocapsid protein (1)
- SEM (1)
- Salinität (1)
- Saliva (1)
- Sample treatment (1)
- Samples (1)
- Screen-printed electrode (1)
- Screeningverfahren (1)
- Selektivität (1)
- Sensing (1)
- Sensitized magnetic nanocatalysts (1)
- Sensors (1)
- Sewage treatment plant (1)
- Singlet oxygen (1)
- Singulettsauerstoff (1)
- Small molecule detection (1)
- Soil amendment (1)
- Soils (1)
- Solid-phase extraction (1)
- Solid-phase synthesis (1)
- Sonochemical synthesis (1)
- Square-wave voltammetry (1)
- Stability (1)
- Structure (1)
- Surface labelled lyophilized PBMC (1)
- Surface plasmon resonance spectroscopy (1)
- Surface waters (1)
- Suspension Array (1)
- TATP (1)
- TEM (1)
- Temperature effects (1)
- Therapeutic Drug Monitoring (1)
- Thin film (1)
- Thiram (1)
- TiO2 (1)
- Toxicology (1)
- Transformationsprodukte (1)
- Triacetone triperoxide (1)
- Trinkwasser (1)
- Two electrophoretic forms (1)
- UV Bestrahlung (1)
- Umweltanalytik (1)
- Urin (1)
- Wasseranalytik (1)
- Waste-water (1)
- Wastewater marker (1)
- Wastewater treatment (1)
- Wastewater treatment plant (1)
- Water (1)
- Water analysis (1)
- Water samples (1)
- Weichmacher (1)
- Zearalenone (1)
- Zebrafisch (1)
- Zeolithe (1)
- Zyklisierung (1)
- antibodies (1)
- caffeine (1)
- microfluidics (1)
- pH-Abhängigkeit (1)
- Östrogene (1)
- β-Lactam (1)
- β-lactam (1)
Organisationseinheit der BAM
- 1 Analytische Chemie; Referenzmaterialien (70)
- 1.8 Umweltanalytik (70)
- 6 Materialchemie (8)
- 1.9 Chemische und optische Sensorik (7)
- 6.1 Oberflächen- und Dünnschichtanalyse (5)
- P Präsident (5)
- P.0 Präsident und andere (5)
- 1.5 Proteinanalytik (3)
- 6.3 Strukturanalytik (3)
- 1.0 Abteilungsleitung und andere (2)
Paper des Monats
- ja (1)
Eingeladener Vortrag
- nein (49)
The title compound [systematic name: 6-(1,3-dimethyl-2,6-dioxo-2,3,6,7-tetrahydro-1H-purin-7-yl)hexanoic acid monohydrate, CAS 61444-23-3], C13H18N4O4·H2O, was synthesized and crystallized from ethyl acetate. Hydrogen bonds between xanthine and water molecules contribute to the formation of layers parallel to (10).
Monitoring carbamazepine in surface and wastewaters by an immunoassay based on a monoclonal antibody
(2009)
The pharmaceutical compound carbamazepine (CBZ) is an emerging pollutant in the aquatic environment and may potentially be used as a wastewater marker. In this work, an enzyme-linked immunosorbent assay (ELISA) for the detection of carbamazepine in surface and sewage waters has been developed. The heterogeneous immunoassay is based on a commercially available monoclonal antibody and a novel enzyme conjugate (tracer) that links the hapten via a hydrophilic peptide (triglycine) spacer to horseradish peroxidase. The assay achieves a limit of detection of 24 ng/L and a quantitation range of 0.05-50 µg/L. The analytical performance and figure of merits were compared to liquid chromatography-tandem mass spectrometry after solid-phase extraction. For nine Berlin surface water samples and one wastewater sample, a close correlation of results was observed. A constant overestimation relative to the CBZ concentration of approximately 30% by ELISA is probably caused by the presence of 10,11-epoxy-CBZ and 2-hydroxy-CBZ in the samples. The ELISA displayed cross-reactivities for these compounds of 83% and 14%, respectively. In a first screening of 27 surface water samples, CBZ was detected in every sample with concentrations between 0.05 and 3.2 µg/L. Since no sample cleanup is required, the assay allowed for the determination of carbamazepine with high sensitivity at low costs and with much higher throughput than with conventional methods.
The aim of this work was to investigate the effects of long-term application of different organic fertilizers (sewage sludge, farmyard manure, compost) as compared to mineral fertilizer on the structure of the soil organic matter. Capillary electrophoresis was employed for the quantification of monosaccharides and phenolic compounds, whereas NMR and FT-IR were used for the overall characterization of the soils organic matter. Application of farmyard manure results in a higher content of organic matter derived from angiosperms, suggested by the higher levels of syringic and vanillic phenols. Spectroscopic studies show an increase of lignin and lignin-like products in the organic matter of the soil, which may be derived from the cereal straw supplied with farmyard manure. According to spectroscopic analysis, 13C CPMAS-NMR and FT-IR spectra, higher contents of methylene groups ( CH2) from proteins and protein-like compounds, as well as higher levels of carbohydrates, were found in the soil supplied with compost. The monosaccharide (rhamnose, xylose, glucose, mannose, arabinose, fucose and galactose) content was not significantly influenced by the different organic fertilizers, suggesting that the type of amendment used does not affect any of these six studied monomers. Comparing the three organic amendments the most significant differences were observed after long-term application of farmyard manure, with an increase in lignin and lignin-like products in the soil organic matter, and compost, which appears to contribute to an increase of protein and protein-like, as well as carbohydrates content on soil organic matter. The knowledge of such changes can be essential to understand the sorption and bioavailability of pollutants, as well as establish/unestablish ways to recycling organic residues as organic fertilizers.