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Autor

  • Brangsch, J. (4)
  • Hamm, B. (4)
  • Kaufmann, Jan O. (4)
  • Makowski, M. R. (4)
  • Adams, L. C. (3)
  • Reimann, C. (3)
  • Botnar, R. M. (2)
  • Buchholz, R. (2)
  • Kader, A. (2)
  • Karst, U. (2)
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Erscheinungsjahr

  • 2021 (1)
  • 2020 (1)
  • 2019 (2)

Schlagworte

  • Magnetic resonance imaging (3)
  • Extracellular matrix (2)
  • Gadolinium (2)
  • Aneurism (1)
  • Diagnosis (1)
  • ESMA (1)
  • Elastin (1)
  • Elastin-specific molecular agent (1)
  • Ferumoxytol (1)
  • Hepatocellular carcinoma (1)
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Organisationseinheit der BAM

  • 1 Analytische Chemie; Referenzmaterialien (4)
  • 1.5 Proteinanalytik (4)

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Molecular MR Imaging of Prostate Cancer (2021)
Kader, A. ; Brangsch, J. ; Kaufmann, Jan O. ; Zhao, J. ; Mangarova, D. B. ; Moeckel, J. ; Adams, L. C. ; Sack, I. ; Taupitz, M. ; Hamm, B. ; Makowski, M. R.
This review summarizes recent developments regarding molecular imaging markers for magnetic resonance imaging (MRI) of prostate cancer (PCa). Currently, the clinical standard includes MR imaging using unspecific gadolinium-based contrast agents. Specific molecular probes for the diagnosis of PCa could improve the molecular characterization of the tumor in a non-invasive examination. Furthermore, molecular probes could enable targeted therapies to suppress tumor growth or reduce the tumor size.
Assessment of the hepatic tumor extracellular matrix using elastin‑specific molecular magnetic resonance imaging in an experimental rabbit cancer model (2020)
Keller, S. ; Borde, T. ; Brangsch, J. ; Reimann, C. ; Kader, A. ; Schulze, D. ; Buchholz, R. ; Kaufmann, Jan O. ; Karst, U. ; Schellenberger, E. ; Hamm, B. ; Makowski, M. R.
To investigate the imaging performance of an elastin-specific molecular magnetic resonance imaging (MRI) probe with respect to the extracellular matrix (ECM) in an experimental hepatic cancer model. Twelve rabbits with hepatic VX2 tumors were examined using 3 T MRI 14, 21, and 28 days after tumor implantation for two subsequent days (gadobutrol, day 1; elastin-specific probe, day 2). The relative enhancement (RE) of segmented tumor regions (central and margin) and the peritumoral matrix was calculated using pre-contrast and delayed-phase T1w sequences. MRI measurements were correlated to histopathology and element-specific and spatially resolved mass spectrometry (MS). Mixed-model analysis was performed to assess the performance of the elastin-specific probe. In comparison to gadobutrol, the elastin probe showed significantly stronger RE, which was pronounced in the tumor margin (day 14–28: P ≤ 0.007). In addition, the elastin probe was superior in discriminating between tumor regions (χ2(4) = 65.87; P < 0.001). MRI-based measurements of the elastin probe significantly correlated with the ex vivo elastinstain (R = .84; P <0 .001) and absolute gadolinium concentrations (ICP-MS: R = .73, P <0 .01). LA-ICP-MS imaging confirmed the colocalization of the elastin-specific probe with elastic fibers. Elastin-specific molecular MRI is superior to non-specific gadolinium-based contrast agents in imaging the ECM of hepatic tumors and the peritumoral tissue.
Dual-probe molecular MRI for the in vivo characterization of atherosclerosis in a mouse model: Simultaneous assessment of plaque inflammation and extracellular matrix remodeling (2019)
Reimann, C. ; Brangsch, J. ; Kaufmann, Jan O. ; Adams, L. C. ; Onthank, D. C. ; Thöne-Reineke, C. ; Robinson, S. P. ; Hamm, B. ; Botnar, R. M. ; Makowski, M. R.
Molecular MRI is a promising in-vivo modality to detect and quantify morphological and molecular vessel-wall changes in atherosclerosis. The combination of different molecular biomarkers may improve the risk stratification of patients. This study aimed to investigate the feasibility of simultaneous visualization and quantification of plaque-burden and inflammatory activity by dual-probe molecular MRI in a mouse-model of progressive atherosclerosis and in response-to-therapy. Homozygous apolipoprotein E knockout mice (ApoE−/−) were fed a high-fat-diet (HFD) for up to four-months prior to MRI of the brachiocephalic-artery. To assess response-to-therapy, a statin was administered for the same duration. MR imaging was performed before and after administration of an elastin-specific gadolinium-based and a macrophage-specific iron-oxide-based probe. Following in-vivo MRI, samples were analyzed using histology, immunohistochemistry, inductively-coupled-mass-spectrometry and laser-inductively-coupled-mass-spectrometry. In atherosclerotic-plaques, intraplaque expression of elastic-fibers and inflammatory activity were not directly linked. While the elastin-specific probe demonstrated the highest accumulation in advanced atherosclerotic-plaques after four-months of HFD, the iron-oxide-based probe showed highest accumulation in early atherosclerotic-plaques after two months of HFD. In-vivo measurements for the elastin and iron-oxide-probe were in good agreement with ex-vivo histopathology (Elastica-van-Giesson stain: y = 298.2 + 5.8, R2 = 0.83, p < 0.05; Perls‘ Prussian-blue-stain: y = 834.1 + 0.67, R2 = 0.88, p < 0.05). Contrast-to-noise-ratio (CNR) measurements of the elastin probe were in good agreement with ICP-MS (y = 0.11x-11.3, R² = 0.73, p < 0.05). Late stage atherosclerotic-plaques displayed the strongest increase in both CNR and gadolinium concentration (p < 0.05). The gadolinium probe did not affect the visualization of the iron-oxide-probe and vice versa. This study demonstrates the feasibility of simultaneous assessment of plaque-burden.
Concurrent Molecular Magnetic Resonance Imaging of Inflammatory Activity and Extracellular Matrix Degradation for the Prediction of Aneurysm Rupture (2019)
Brangsch, J. ; Reimann, C. ; Kaufmann, Jan O. ; Adams, L. C. ; Onthank, D. C. ; Thöne-Reinecke, C. ; Robinson, S. P. ; Buchholz, R. ; Karst, U. ; Botnar, R. M. ; Hamm, B. ; Makowski, M. R.
This study demonstrates the potential of the concurrent assessment of inflammatory activity and ECM degradation by dual-probe molecular MRI in an experimental mouse model of AAA. Based on the combined information from both molecular probes the rupture of AAAs could reliably be predicted, with higher accuracy compared with each probe alone. The combined in vivo quantification of these biomarkers in 1 imaging session may be useful to improve the in vivo characterization of AAAs.
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