Filtern
Erscheinungsjahr
- 2021 (3) (entfernen)
Dokumenttyp
Sprache
- Englisch (3)
Schlagworte
- Core-shell particles (2)
- Electron microscopy (2)
- Image processing (2)
- Roughness (2)
- Amperometry (1)
- Cyclic voltammetry (1)
- Electrochemistry (1)
- Immunoassay (1)
- MamaLoCA (1)
- MamaLoCa (1)
- Ochratoxin A (1)
- Particle Characterization (1)
- Particle characterisation (1)
Organisationseinheit der BAM
Electrochemical methods offer great promise in meeting the demand for user-friendly on-site devices for Monitoring important parameters. The food industry often runs own lab procedures, for example, for mycotoxin analysis, but it is a major goal to simplify analysis, linking analytical methods with smart technologies. Enzyme-linked immunosorbent assays, with photometric detection of 3,3’,5,5’-tetramethylbenzidine (TMB),form a good basis for sensitive detection. To provide a straightforward approach for the miniaturization of the detectionstep, we have studied the pitfalls of the electrochemical TMB detection. By cyclic voltammetry it was found that the TMB electrochemistry is strongly dependent on the pH and the electrode material. A stable electrode response to TMB could be achieved at pH 1 on gold electrodes. We created a smartphonebased, electrochemical, immunomagnetic assay for the detection of ochratoxin A in real samples, providing a solid basis forsensing of further analytes.
A particle roughness analysis tool, based on electron microscopy images (SEM and TEM). The influence of various parameters on the calculated roughness was also investigated: the setting of the proper threshold, accelerating voltage, etc. The samples were gradually tilted to extend imaging information of more than only one projection. Furthermore, the measurement uncertainty of the profile roughness of particles associated to various orientations was estimated.