Filtern
Erscheinungsjahr
Dokumenttyp
- Zeitschriftenartikel (29)
- Posterpräsentation (14)
- Vortrag (5)
- Beitrag zu einem Tagungsband (2)
Schlagworte
- Fluorescence (14)
- Nanoparticles (9)
- Nanoparticle (6)
- Quantum yield (6)
- NIR (5)
- Dye (4)
- Microparticles (3)
- AIE (2)
- Brightness (2)
- Cancer (2)
- Cell imaging (2)
- Coumarin (2)
- Encapsulation (2)
- FLIM (2)
- Fluorescence lifetime imaging microscopy (2)
- Fluorescence standard (2)
- Glass (2)
- Integrating sphere spectroscopy (2)
- Lifetime (2)
- Lifetime multiplexing (2)
- Multiplexing (2)
- Near-infrared (2)
- Photoluminescence (2)
- Polystyrene (2)
- Probe (2)
- Quality assurance (2)
- Quantification (2)
- Quantum dot (2)
- Rhodamine (2)
- Spectroscopy (2)
- Standard (2)
- Surface analysis (2)
- Surface groups (2)
- Synthesis (2)
- Viscosity (2)
- Absolute flourometry (1)
- Absolute fluorescence quantum yield (1)
- Aggregate (1)
- Aggregates (1)
- Aggregation (1)
- Aldrithiol (1)
- Alexa dyes (1)
- Allergic airway inflammation (1)
- Alveolar macrophages (1)
- BCA assay (1)
- Biotin–FITC titration (1)
- CIEE (1)
- CLSM (1)
- Calibration (1)
- Calibration tool (1)
- Cell tracking (1)
- Cleavable linker (1)
- Cleavable reporter (1)
- Cross section (1)
- Cyanine dye (1)
- Cytotoxicity (1)
- Dimer (1)
- Dye content (1)
- Dye-doped polymers (1)
- Ellman (1)
- Ellman's test (1)
- Emission standards (1)
- Environment (1)
- Fluorescence lifetime imaging (1)
- Fluorescence lifetime imaging FLIM (1)
- Fluorescent label (1)
- Fluorescent particles (1)
- Fluorophore (1)
- IR (1)
- Imaging (1)
- In vitro test (1)
- In vivo imaging (1)
- In vivo near-infrared fluorescence imaging (1)
- In vivo test (1)
- Indolone merocyanines (1)
- Instrument performance validation (1)
- Intensity standard (1)
- Itrybe nanoparticles (1)
- Label (1)
- Laponite (1)
- Laser dye (1)
- Lifetime Multiplexing (1)
- Ligand analysis (1)
- Ligands (1)
- Liquid standard (1)
- Lluminescence (1)
- Method (1)
- Multiparametric (1)
- NIR dyes (1)
- Nano particles (1)
- Nanobiotechnology (1)
- Nanocavity (1)
- Nanoclay (1)
- Near infrared (1)
- Nile Red (1)
- Nile red (1)
- Optical (1)
- Optical probe (1)
- Optical quantification (1)
- Organic dye (1)
- Oxygene (1)
- PEG quantification (1)
- PMMA (1)
- Particle (1)
- Particle Label (1)
- Particles (1)
- Perylene (1)
- Photoacoustic spectroscopy (1)
- Photometric assay (1)
- Photostability (1)
- Polarity (1)
- Polarity probe (1)
- Polyethylene glycol (1)
- Polymer (1)
- Polymer particles (1)
- Polystyrene nanoparticles (1)
- Protective shell (1)
- Protein quantification (1)
- Quantum dots (1)
- Quantum yield standard (1)
- Quantum yields (1)
- Reference material (1)
- Rhodamine 800 (1)
- Silica (1)
- Size (1)
- Spectral fluorescence standard (1)
- Surface modification (1)
- Swelling (1)
- Syntheseis (1)
- Thermal lensing (1)
- Traceability (1)
- Up-conversion nanocrystal (1)
- Validation (1)
- Zeta potential (1)
- encoding (1)
- silica nanoparticles (1)
- surface and in-depth inspection (1)
- titania nanoparticles (1)
Organisationseinheit der BAM
Eingeladener Vortrag
- nein (5)
Targeted ratiometric Nanosensors for the in Vivo Imaging of Tumor Hypoxia in the NIR spectral region
(2012)
Dye-biomolecule conjugates and NIR-fluorescent particles for targeting of disease-related biomarkers
(2011)
Indispensable for fluorescence imaging are highly specific and sensitive molecular probes that absorb and emit in the near infrared (NIR) spectral region and respond to or target molecular species or processes. Here, we present approaches to targeted fluorescent probes for in vivo imaging in the intensity and lifetime domain exploiting NIR dyes. Screening schemes for the fast identification of suitable fluorophores are derived and design criteria for highly emissive optical probes. In addition, as a signal amplification strategy that enables also the use of hydrophobic NIR fluorophores as fluorescent reporters, first steps towards versatile strategies for the preparation of NIR-fluorescent polymeric particles are presented that can be utilized also for the design of targeted and analyte-responsive probes.
Polystyrene nanoparticles (PS-NPs) were doped with an oxygen-sensitive near-infrared (NIR)-emissive palladium meso-tetraphenylporphyrin and an inert reference dye which are both excitable at 635 nm. The nanosensors were characterized with special emphasis on fundamental parameters such as absolute photoluminescence quantum yield and fluorescence lifetime. The PS-NPs were employed for ratiometric dual-wavelength and lifetime-based photoluminescent oxygen sensing. They were efficiently taken up by cultured murine alveolar macrophages, yielding a characteristic and reversible change in ratiometric response with decreasing oxygen concentration. This correlated with the cellular hypoxic status verified by analysis of hypoxia inducible factor-1α (HIF-1α) accumulation. In addition, the surface of PS-NPs was functionalized with polyethylene glycol (PEG) and the monoclonal antibody herceptin, and their binding to HER2/neu-overexpressing tumor cells was confirmed in vitro. First experiments with tumor-bearing mouse revealed a distinctive ratiometric response within the tumor upon hypoxic condition induced by animal sacrifice. These results demonstrate the potential of these referenced NIR nanosensors for in vitro and in vivo imaging that present a new generation of optical probes for oncology.
Photoluminescence techniques are amongst the most widely used tools in the material and life sciences, with new and exciting applications continuously emerging. Advantages include their comparative ease of use, unique sensitivity, non-invasive character, and potential for multiplexing, remote sensing, and miniaturization. Increasing applications of fluorescence techniques in the life sciences and emerging medical applications of fluorescence microscopic techniques including 1P and 2P fluorescence microscopy combined with fluorescence lifetime imaging (FLIM) in e.g. in vivo eye diagnostics boosted the demand for robust, easy-to-use, and reliable fluorescence standards to ensure the reliability and comparability of fluorescence data. This includes fluorescence standards for the consideration of instrument-specific spectral and intensity distortions of measured signals and instrument performance validation, fluorescence intensity standards for the quantification of measured intensities and for signal referencing, and lately, also fluorescence lifetime standards.
Synthesis and characterisation of highly fluorescent core-shell nanoparticles based on Alexa dyes
(2012)
Current and future developments in the
emerging field of nanobiotechnology are closely
linked to the rational design of novel fluorescent
nanomaterials, e.g. for biosensing and imaging applications.
Here, the synthesis of bright near infrared
(NIR)-emissive nanoparticles based on the grafting
of silica nanoparticles (SNPs) with 3-aminopropyl
triethoxysilane (APTES) followed by covalent attachment
of Alexa dyes and their subsequent shielding by an
additional silica shell are presented. These nanoparticles
were investigated by dynamic light scattering (DLS),
transmission electron microscopy (TEM) and fluorescence
spectroscopy. TEM studies revealed the monodispersity
of the initially prepared and fluorophorelabelled
silica particles and the subsequent formation of
raspberry-like structures after addition of a silica
precursor.Measurements of absolute fluorescence quantum
yields of these scattering particle suspensions with
an integrating sphere setup demonstrated the influence
of dye labelling density-dependent fluorophore aggregation
on the signaling behaviour of such nanoparticles.