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- 2019 (2) (entfernen)
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- Englisch (2) (entfernen)
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- Holographic microscopy (1)
- MIPs (1)
- Macrophages (1)
- Sialic acid (1)
- agricultural economy (1)
- embedded sensor (1)
- environment (1)
- fluorescence (1)
- gas analysis (1)
- spectroscopy (1)
Organisationseinheit der BAM
This paper describes the development of a semi-automatic gas measurement device presenting potentially a broad range of applications, noteworthy in the agricultural sector. Non-reversible fluorescent molecular sensors were designed and syn-thesized. Upon, integration into a hydrogel matrix with an optimal ratio of co-solvents, the sensors reacting selectively to ammonia were illuminated by excitation light to produce a concentration-correlated fluorescence emission. An automated mechanical-elec-trical device initiates a given gas mixture and thus simulates con-centrations similar to a threshold value. The aim of this project is to develop a sensor or a low-cost method which can monitor low concentrations of harmful gases and aid in their elimination or regulation in livestock housing, barns or stables.
Digital holographic cytometry (DHC) is a state-of-the-art quantitative Phase imaging (QPI) method that permits time-lapse imaging of cells without induced cellular toxicity. DHC platforms equipped with semi-automated image segmentation and analysis software packages for assessing cell behavior are commercially available. In this study we investigate the possible uptake of nanoprobes in macrophages in vitro over time.