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- Englisch (2)
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- AFM (1)
- Atomic Force Microscopy (1)
- CdSe/ZnS quantum Dots (1)
- Cellular uptake (1)
- Elemental Distribution (1)
- Fast single pulse response (1)
- HT22 (1)
- HeLa (1)
- LA-ICP-SFMS (1)
- Laser Ablation (1)
Organisationseinheit der BAM
- 9 Komponentensicherheit (2) (entfernen)
High spatially resolved quantitative bioimaging of CdSe/ZnS Quantum Dots uptake in two kinds of cells is investigated combining laser ablation inductively coupled plasma mass spectrometry and the spatially resolved analysis of dried pL-droplets from a solution with a known concentration of Quantum Dots. Single cells and dried pL-droplets are morphologically characterized by Atomic Force Microscopy. A number concentration of CdSe/ZnS QDs between 3.5 104 and 48 104 is estimated to be uptaken by several selected single cells, after being incubated in the presence of a QDs suspension added to a standard cell culture medium. Mono-elemental bioimaging at subcellular resolution seems to show a higher number concentration of the CdSe/ZnS QDs in the cytosol around the cell nucleus.
The analytical potential of a nanosecond laser ablation coupled plasma mass spectrometer (ns-LA-ICP-SFMS)system is investigated for fast and highly spatially resolved (~µm) elemental distribution within single cells. The size, morphology and overlapping of laser-induced craters has been investigated with Atomic Force Microscopy (AFM).