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Glycosylation is heavily altered in tumor cells compared with healthy cells, because of the different levels of expression of glycosyltransferases, glycosidases and monosaccharide transporters within a cancerous microenvironment. 1 Tumor-associated glycans, especially sialic acid (SA) conjugates, which are good candidates of tumor markers, can be used for Cancer early diagnosis. 2 However, studies on SA-binding lectins demonstrate that the type of SAlinkage and the glycosylation position of carbohydrate can greatly influence the affinity. 3 Therefore, there is a need for diagnostic tools owning high specificity and strong affinity to analyze and determine SA glycosylation motifs. 4 Herein, we report the development of fluorescent core/shell/shell nanoparticles where the outermost layer is a thin molecularly imprinted polymer (MIP) film binding to tumor cells in vitro by targeting cell surface SA. The core is made of N-doped red carbon nanodots (R-CNDs) which were prepared by hydrothermal synthesis, emitting intense red fluorescence under fluorescence microscopy imaging conditions. Silica coating of R-CNDs was achieved by a microemulsion method, resulting in ca. 40 nm large silica-coated CNDs (named R-CSNs).
Finally, a thin MIP-shell was accomplished by using a combination of non-fluorescent
monomer (3), functional monomers (1, 2) and cross-linker (EGDMA) for polymerization
(Figure 1). Transmission electron microscopy (TEM) is used for structural characterization of
the formation of MIP layer, and the results of cell-based binding tests show a promising binding
affinity of MIPs to tumor cells, allowing a relatively robust, specific and rapid analytical
method for cancer biopsies.