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SETNanoMetro, a European Seventh Framework project, seeks to develop standard synthetic routes and metrological characterisation methods for the development and production of TiO₂ nanoparticles and nano-sized coatings with highly-defined, homogeneous and reproducible characteristics, including bulk structure, size, shape and surface structure. These materials are being tested for their potential in selected technological applications, including as coatings on bone-substituting prostheses. The objective of this study was to assess how variations in morphology and chemistry of nano-sized TiO₂ coatings affect their biocompatibility in vitro.