Evaluating the Normalization Potential of Anthropogenic Markers in Wastewater: Monitoring Influents of German Treatment Plants by ELISA
- Wastewater-based epidemiology (WBE) has emerged as a vital tool for pandemic preparedness, offering early warning capabilities and supporting public health interventions. In Germany, combined sewer systems are prevalent, leading to fluctuations in wastewater volume and com-position due to rainwater inflow, especially through street drains. This variability complicates the quantitative measurement of pathogens and pollutants in wastewater. While human excretion provides constant inputs, these parameters are often non-specific, lost, or degraded during transport. Pharmaceuticals—such as carbamazepine, diclofenac, and clarithromycin—as well as commonly consumed substances like caffeine, exhibit sufficiently high concentrations and good chemical stability in wastewater and are not subject to seasonal fluctuations.
Antibody-based methods, particularly Enzyme-Linked Immunosorbent Assays (ELISA), offer a cost-effective alternative to complex chromatographic techniques for monitoringWastewater-based epidemiology (WBE) has emerged as a vital tool for pandemic preparedness, offering early warning capabilities and supporting public health interventions. In Germany, combined sewer systems are prevalent, leading to fluctuations in wastewater volume and com-position due to rainwater inflow, especially through street drains. This variability complicates the quantitative measurement of pathogens and pollutants in wastewater. While human excretion provides constant inputs, these parameters are often non-specific, lost, or degraded during transport. Pharmaceuticals—such as carbamazepine, diclofenac, and clarithromycin—as well as commonly consumed substances like caffeine, exhibit sufficiently high concentrations and good chemical stability in wastewater and are not subject to seasonal fluctuations.
Antibody-based methods, particularly Enzyme-Linked Immunosorbent Assays (ELISA), offer a cost-effective alternative to complex chromatographic techniques for monitoring anthropogenic markers. They demonstrate superior normalization quality compared to the frequently used quantitative PCR-based marker Pepper mild mottle virus (PMMoV).…


| Autor*innen: | Rudolf J. SchneiderORCiD |
|---|---|
| Koautor*innen: | Zoltán KonthurORCiD, Maximilian Lehnert, Shireen Ewald, Caitlin Hartlage, Doğa İkizoğulları, Thomas Exner, Marcus Lukas, Ulrike BraunORCiD |
| Dokumenttyp: | Posterpräsentation |
| Veröffentlichungsform: | Präsentation |
| Sprache: | Englisch |
| Jahr der Erstveröffentlichung: | 2025 |
| Organisationseinheit der BAM: | 1 Analytische Chemie; Referenzmaterialien |
| 1 Analytische Chemie; Referenzmaterialien / 1.8 Umweltanalytik | |
| DDC-Klassifikation: | Technik, Medizin, angewandte Wissenschaften / Ingenieurwissenschaften / Sanitär- und Kommunaltechnik; Umwelttechnik |
| Freie Schlagwörter: | Carbamazepin; Koffein; Spurenstoffe Anthropogener Marker Immunoassay |
| Themenfelder/Aktivitätsfelder der BAM: | Umwelt |
| Veranstaltung: | 6th International Conference on Risk Assessment of Pharmaceuticals in the Environment - ICRAPHE |
| Veranstaltungsort: | Aveiro, Portugal |
| Beginndatum der Veranstaltung: | 20.10.2025 |
| Enddatum der Veranstaltung: | 21.10.2025 |
| Verfügbarkeit des Dokuments: | Datei im Netzwerk der BAM verfügbar ("Closed Access") |
| Datum der Freischaltung: | 22.10.2025 |
| Referierte Publikation: | Nein |

