Metal-Organic Magnetic Framework-Based Immunosensor for Label-free Electrochemical sensing of NeuroToxin

  • A metal-organic framework (MOF)-integrated microfluidic flow-cell (MFC) based electrochemical immunodetection of the tetanus toxoid (TT) is presented. The magnetic property of Fe-MOF helped to hold them on the working electrode (WE) at detection zone of MFC, surpassing the requirement of additional conjugation chemistry, and the conductive property was utilized to observe the change in signal efficiency in the presence of TT. The one-pot hydrothermal synthesis of iron-based MOF (Fe-MOF) was performed using the Fe3+/Fe2+ precursors as 1.2/1 mmol and dual ligands, tetrahydroxy-1,4-benzoquinone and 2 aminobenzene-1,4-dicarboxylic acid. The human anti-TT mAb SA13 was purified in-house from the cell culture supernatant of SA13 hybridoma cells by Protein-A affinity. SDS-PAGE showed the high purity as of a band just above the 140kDa mark in the eluate lane. The hydrodynamic radius of 5.35 nm and thermal unfolding temperature of 73°C were typical for antibodies. Indirect ELISA confirmed theA metal-organic framework (MOF)-integrated microfluidic flow-cell (MFC) based electrochemical immunodetection of the tetanus toxoid (TT) is presented. The magnetic property of Fe-MOF helped to hold them on the working electrode (WE) at detection zone of MFC, surpassing the requirement of additional conjugation chemistry, and the conductive property was utilized to observe the change in signal efficiency in the presence of TT. The one-pot hydrothermal synthesis of iron-based MOF (Fe-MOF) was performed using the Fe3+/Fe2+ precursors as 1.2/1 mmol and dual ligands, tetrahydroxy-1,4-benzoquinone and 2 aminobenzene-1,4-dicarboxylic acid. The human anti-TT mAb SA13 was purified in-house from the cell culture supernatant of SA13 hybridoma cells by Protein-A affinity. SDS-PAGE showed the high purity as of a band just above the 140kDa mark in the eluate lane. The hydrodynamic radius of 5.35 nm and thermal unfolding temperature of 73°C were typical for antibodies. Indirect ELISA confirmed the high binding affinity. The mAb was conjugated on the Fe-MOF/Phe surface with the help of ethylenediamine (Fe MOF/Phe/EDA/anti-TTmAb). A specifically tailored MFC, made of Poly(methyl methacrylate) substrate was crafted at the BAM workshop. The limit of detection (LOD) and analytical sensitivity of the TT biosensor was 9.4 ng/ml [y=1.93E-5 + (-1.86E-6)x] and 11.6 μA/ng ml-1cm-2, respectively. The good reproducibility, selectivity, and stability show the applicability of these Fe-MOFs for detecting various other toxins.zeige mehrzeige weniger

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Autor*innen:Sarita DeviORCiD
Koautor*innen:Soraya RiedelORCiD, Sarah DöringORCiD, Lukas Hiller, Archana Kaliyaraj Selva KumarORCiD, Sabine Flemig, Chandan SinghORCiD, Zoltán KonthurORCiD, Vasile-Dan HodoroabaORCiD, Rudolf SchneiderORCiD
Dokumenttyp:Posterpräsentation
Veröffentlichungsform:Präsentation
Sprache:Englisch
Jahr der Erstveröffentlichung:2025
Organisationseinheit der BAM:1 Analytische Chemie; Referenzmaterialien
1 Analytische Chemie; Referenzmaterialien / 1.8 Umweltanalytik
DDC-Klassifikation:Naturwissenschaften und Mathematik / Chemie / Analytische Chemie
Freie Schlagwörter:Antibody; Biosensor; Electrochemistry; Magnetic Metal-Organic Framework; Tetanus toxoid
Themenfelder/Aktivitätsfelder der BAM:Chemie und Prozesstechnik
Chemie und Prozesstechnik / Chemische Charakterisierung und Spurenanalytik
Veranstaltung:European Materials Research Society (E-MRS) Spring Meeting
Veranstaltungsort:Strasbourg, France
Beginndatum der Veranstaltung:26.05.2025
Enddatum der Veranstaltung:30.05.2025
Verfügbarkeit des Dokuments:Datei im Netzwerk der BAM verfügbar ("Closed Access")
Datum der Freischaltung:26.06.2025
Referierte Publikation:Nein
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