Inflammation-related key events stimulated by micro- and nanoplastics

  • The presence of micro- and nanoplastic particles (MNP) in our environment has raised increasing public and political concern. Increasing evidence suggests that humans are exposed to these MNP, mostly via inhalation or ingestion. The EC-Horizon project POLYRISK (https://polyrisk.science) aims to establish an IATA/AOP-based tiered approach to assess human health risks of MNP. In our research, we focus on assessing immunotoxicological effects of MNP. We have tested a series of primary, secondary, environmentally aged (incubated in River Rine water) and chemically altered MNP for their potential to affect dendritic cells or macrophages. MNP used in this study include primary and weathered PS, secondary PVC, PA, PP (with or without talc) and PE. The secondary PE and PP (w/o talc) particles appeared to have oxidized groups on their surface. Data shows that MNP can be engulfed by macrophages (PMA-stimulated human THP1 cells) and human blood-derived dendritic cells (DCs). The effects ofThe presence of micro- and nanoplastic particles (MNP) in our environment has raised increasing public and political concern. Increasing evidence suggests that humans are exposed to these MNP, mostly via inhalation or ingestion. The EC-Horizon project POLYRISK (https://polyrisk.science) aims to establish an IATA/AOP-based tiered approach to assess human health risks of MNP. In our research, we focus on assessing immunotoxicological effects of MNP. We have tested a series of primary, secondary, environmentally aged (incubated in River Rine water) and chemically altered MNP for their potential to affect dendritic cells or macrophages. MNP used in this study include primary and weathered PS, secondary PVC, PA, PP (with or without talc) and PE. The secondary PE and PP (w/o talc) particles appeared to have oxidized groups on their surface. Data shows that MNP can be engulfed by macrophages (PMA-stimulated human THP1 cells) and human blood-derived dendritic cells (DCs). The effects of virgin as well as secondary MNP (0, 10 or 100 μg/ml) on THP1 macrophages were limited to decrease of mitochondrial activity (Alamar blue), increase of cellular leakage (LDH), and stimulation of lysosomal activity. None of these MNP stimulated gene expression of NFκB or release of pro-inflammatory cytokines (IL-6, TNFα, IL-1β). On the other hand, secondary PP and PE with oxidized surface groups did increase NFκB gene expression and release of cytokines by THP1 macrophages. Virgin and weathered PS particles were tested using DCs, and only weathered PS did stimulate DC activity (increased costimulatory molecules CD83, CD86) and as consequence allogeneic T cells. DC activation appeared to result from environmental contaminants. In conclusion, of all MNPs tested only those that contained active surface groups or environmental components appeared to be immunostimulatory, whereas primary and secondary MNP rather reduced macrophage activity and viability (at relatively high concentrations of 100 μg/ml). Further research is needed to reveal molecular mechanisms and to translate in vitro findings to real-world exposure scenarios.zeige mehrzeige weniger

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Autor*innen:A. van den Berg, K. Adriaans, E. Hoppener, L. Parker, A. Boersma, Korinna AltmannORCiD, J. Legler, R. Pieters
Dokumenttyp:Vortrag
Veröffentlichungsform:Präsentation
Sprache:Englisch
Jahr der Erstveröffentlichung:2024
Organisationseinheit der BAM:6 Materialchemie
6 Materialchemie / 6.5 Synthese und Streuverfahren nanostrukturierter Materialien
DDC-Klassifikation:Technik, Medizin, angewandte Wissenschaften / Ingenieurwissenschaften / Sanitär- und Kommunaltechnik; Umwelttechnik
Freie Schlagwörter:Human health risks; Immunotoxicological effects; Micro- and nanoplastic particles (MNP)
Themenfelder/Aktivitätsfelder der BAM:Umwelt
Umwelt / Umwelt-Material-Interaktionen
Veranstaltung:58th Congress of the European Societies of Toxicology (EUROTOX 2024)
Veranstaltungsort:Copenhagen, Denmark
Beginndatum der Veranstaltung:08.09.2024
Enddatum der Veranstaltung:11.09.2024
DOI:10.1016/j.toxlet.2024.07.052
Verfügbarkeit des Dokuments:Datei im Netzwerk der BAM verfügbar ("Closed Access")
Datum der Freischaltung:12.06.2025
Referierte Publikation:Nein
Eingeladener Vortrag (wissenschaftliche Konferenzen):Nein
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