LA-MC-ICPMS analysis of Cu isotopes in biological material
- Stable metal isotopes receive increasing attention as medical biomarkers due to their potential to detect changes of the metal metabolism related to disease. Potential mechanisms causing isotope fractionation include biological processes that involve redox- or bond-forming reactions and interaction of metals during transmembrane import and export. In order to advance our understanding of the underlying processes responsible for isotope fractionation between normal and diseased cells, we need in situ, spatially resolved methods. Despite its frequent use, laser ablation - multi-collector - inductively coupled plasma mass spectrometry (LA-MC-ICPMS) analysis of biological material is severely limited by the scarcity of matrix-matched standards. Such matrix-matched standards are necessary to correct for instrumental sources of isotope fractionation such as particle size distribution, ablation physics and differential ionization.
Copper stable isotopes have proven to be a particularlyStable metal isotopes receive increasing attention as medical biomarkers due to their potential to detect changes of the metal metabolism related to disease. Potential mechanisms causing isotope fractionation include biological processes that involve redox- or bond-forming reactions and interaction of metals during transmembrane import and export. In order to advance our understanding of the underlying processes responsible for isotope fractionation between normal and diseased cells, we need in situ, spatially resolved methods. Despite its frequent use, laser ablation - multi-collector - inductively coupled plasma mass spectrometry (LA-MC-ICPMS) analysis of biological material is severely limited by the scarcity of matrix-matched standards. Such matrix-matched standards are necessary to correct for instrumental sources of isotope fractionation such as particle size distribution, ablation physics and differential ionization.
Copper stable isotopes have proven to be a particularly powerful tool to identify differences in isotope composition between tumors and healthy tissue suggesting application in cancer diagnosis [1, 2]. To further our knowledge of Cu isotope fractionation processes induced by diseases we have developed gelatin-based bracketing standards allowing to correct instrumentally induced isotope fractionation during LA-MC-ICPMS analysis. Since gelatin properties resemble properties of protein-rich cellular material, they mimic biological matrices and their ablation behaviour. Hence, gelatin standards are spiked with known amounts of Cu stable isotopes of a known Cu isotope composition and used as matrix-matched bracketing standard. The method achieved reproducibilities of better than 0.15‰ (2SD) for inorganic reference materials and reproducibilities of better than 0.17‰ (2SD) for biological reference materials. The developed routine was tested on a liver tumor model and in situ Cu isotope compositions between healthy (δ65/63Cu = -1.5 to 0.2 ‰) and tumorous (δ65/63Cu = 0.0 to 1.3 ‰) liver tissue could be distinguished [3].…


| Autor*innen: | Mathias SchannorORCiD |
|---|---|
| Koautor*innen: | Marcus OelzeORCiD, Heike TraubORCiD, Yubei He, Robin Schmidt, Lynn Jeanette Savic, Jochen Vogl, Björn Meermann |
| Dokumenttyp: | Vortrag |
| Veröffentlichungsform: | Präsentation |
| Sprache: | Englisch |
| Jahr der Erstveröffentlichung: | 2025 |
| Organisationseinheit der BAM: | 1 Analytische Chemie; Referenzmaterialien |
| 1 Analytische Chemie; Referenzmaterialien / 1.1 Anorganische Spurenanalytik | |
| DDC-Klassifikation: | Naturwissenschaften und Mathematik / Chemie / Analytische Chemie |
| Freie Schlagwörter: | Biomarker; Laser Ablation; Reference Material |
| Themenfelder/Aktivitätsfelder der BAM: | Chemie und Prozesstechnik |
| Chemie und Prozesstechnik / Chemische Charakterisierung und Spurenanalytik | |
| Veranstaltung: | ANAKON 2025 |
| Veranstaltungsort: | Leipzig, Germany |
| Beginndatum der Veranstaltung: | 10.03.2025 |
| Enddatum der Veranstaltung: | 10.03.2025 |
| Verfügbarkeit des Dokuments: | Datei im Netzwerk der BAM verfügbar ("Closed Access") |
| Datum der Freischaltung: | 07.04.2025 |
| Referierte Publikation: | Nein |
| Eingeladener Vortrag (wissenschaftliche Konferenzen): | Nein |

