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Surface characterisation of Escherichia coli under various conditions by near-ambient pressure XPS

  • Bacteria are inherently in a hydrated state and therefore not compatible to ultra-high vacuum techniques such as XPS without prior sample preparation involving freeze drying or fast freezing. This has changed with the development of near-ambient pressure (NAP)-XPS, which makes it possible to characterise the bacterial surface with minimal sample preparation. This paper presents NAP-XPS measurements of Escherichia coli under various NAP conditions: at 11 mbar in a humid environment, at 2 mbar after drying in the chamber, pre-dried at 4 mbar, and at 1 mbar after overnight pumping at 10^−4 mbar. The high-resolution spectra of carbon, nitrogen, and oxygen are presented and found to be in general agreement with XPS measurements from freeze-dried and fast-frozen bacteria. However, it was found that the amount of carbon components associated with polysaccharides increases relative to aliphatic carbon during drying and increases further after overnight pumping. This implies that drying has anBacteria are inherently in a hydrated state and therefore not compatible to ultra-high vacuum techniques such as XPS without prior sample preparation involving freeze drying or fast freezing. This has changed with the development of near-ambient pressure (NAP)-XPS, which makes it possible to characterise the bacterial surface with minimal sample preparation. This paper presents NAP-XPS measurements of Escherichia coli under various NAP conditions: at 11 mbar in a humid environment, at 2 mbar after drying in the chamber, pre-dried at 4 mbar, and at 1 mbar after overnight pumping at 10^−4 mbar. The high-resolution spectra of carbon, nitrogen, and oxygen are presented and found to be in general agreement with XPS measurements from freeze-dried and fast-frozen bacteria. However, it was found that the amount of carbon components associated with polysaccharides increases relative to aliphatic carbon during drying and increases further after overnight pumping. This implies that drying has an impact on the bacterial surface.zeige mehrzeige weniger

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Metadaten
Autor*innen:Marit Kjaervik, Karin SchwibbertORCiD, P. Dietrich, A. Thissen, Wolfgang UngerORCiD
Dokumenttyp:Zeitschriftenartikel
Veröffentlichungsform:Verlagsliteratur
Sprache:Englisch
Titel des übergeordneten Werkes (Englisch):Surface and Interface Analysis
Jahr der Erstveröffentlichung:2018
Veröffentlichende Institution:Bundesanstalt für Materialforschung und -prüfung (BAM)
Verlag:John Wiley & Sons
Verlagsort:Hoboken, New Jersey, USA
Jahrgang/Band:50
Ausgabe/Heft:11
Erste Seite:996
Letzte Seite:1000
DDC-Klassifikation:Naturwissenschaften und Mathematik / Chemie / Analytische Chemie
Freie Schlagwörter:Bacteria; E. coli; NAP-XPS
Veranstaltung:European conference on applications of surface and interface analysis (ECASIA'17)
Veranstaltungsort:Montpellier, France
Beginndatum der Veranstaltung:24.09.2018
Enddatum der Veranstaltung:29.09.2018
DOI:10.1002/sia.6480
URN:urn:nbn:de:kobv:b43-454047
ISSN:0142-2421
ISSN:1096-9918
Verfügbarkeit des Dokuments:Datei für die Öffentlichkeit verfügbar ("Open Access")
Lizenz (Deutsch):License LogoCreative Commons - CC BY-NC-ND - Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International
Datum der Freischaltung:09.07.2018
Referierte Publikation:Ja
Datum der Eintragung als referierte Publikation:15.11.2018
Schriftenreihen ohne Nummerierung:Wissenschaftliche Artikel der BAM
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