A new strategy of reagents labeling (NPs) used in immunoassay with LA-ICP-MS
- Laser ablation with inductively coupled plasma is still more used in life science as biology and biomedicine and the utilization of metals and proteins determination simultaneously is also growing up. We have developed a new strategy of labeling of antibody (it can specific binds to proteins) by nanoparticles and quantum dots which is composed of thousands of atoms and thus increases the sensitivity enormously and of course decreases the Limit of detection, compare to lanthanoids labeling. The ability of successfully tagged antibodies bound to Antigen (protein) was proved by dot blot on membrane imaged by LA-ICP-MS.
Autor*innen: | M. Tvrdoňová, Lena Ascher |
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Koautor*innen: | Norbert Jakubowski, M. Vaculovičová, A. Moravanská, T. Vaněčková, T. Vaculovič |
Dokumenttyp: | Posterpräsentation |
Veröffentlichungsform: | Präsentation |
Sprache: | Englisch |
Jahr der Erstveröffentlichung: | 2017 |
DDC-Klassifikation: | Naturwissenschaften und Mathematik / Chemie / Analytische Chemie |
Freie Schlagwörter: | Immunoassay; LA-ICP-MS; Labeling; Nanoparticle |
Veranstaltung: | Euroanalysis 2017 |
Veranstaltungsort: | Stockholm, Sweden |
Beginndatum der Veranstaltung: | 28.08.2017 |
Enddatum der Veranstaltung: | 01.09.2017 |
Verfügbarkeit des Dokuments: | Datei im Netzwerk der BAM verfügbar ("Closed Access") |
Datum der Freischaltung: | 28.11.2017 |
Referierte Publikation: | Nein |