Multiplex suspension array fluorescence immunoassay (SAFIA) for environmental screening of bioactive compounds in wastewater.

  • Due to the demand of monitoring the water quality regarding emerging pollutants, such as drug residues, selective, high-throughput and multi-target analytical methods must be established. On the one hand, the influence of sewage on natural surface waters must be routinely monitored. On the other hand, estimation of removal efficiencies of pollutants, such as drug residues, is in the focus of industrial and public wastewater treatment. Immunoassays, such as ELISA, offer the possibility to be highly sensitive and selective due to the high target affine and specific recognition of antibodies to target molecules. Batch-wise processing in microtiter plates allow the necessary high-throughput, but only a single analyte can be determined within one measurement. To overcome these disadvantages, we developed a four-plex micro-bead based flow cytometric assay, which is adaptable for the microtiter plate format. The modular and self-prepared bead support consists ofDue to the demand of monitoring the water quality regarding emerging pollutants, such as drug residues, selective, high-throughput and multi-target analytical methods must be established. On the one hand, the influence of sewage on natural surface waters must be routinely monitored. On the other hand, estimation of removal efficiencies of pollutants, such as drug residues, is in the focus of industrial and public wastewater treatment. Immunoassays, such as ELISA, offer the possibility to be highly sensitive and selective due to the high target affine and specific recognition of antibodies to target molecules. Batch-wise processing in microtiter plates allow the necessary high-throughput, but only a single analyte can be determined within one measurement. To overcome these disadvantages, we developed a four-plex micro-bead based flow cytometric assay, which is adaptable for the microtiter plate format. The modular and self-prepared bead support consists of polystyrene-core-silica-shell particles. While the polystyrene core is used for encoding, by introducing different amounts of fluorescent dyes, the silica-shell creates a solid-support for the immunoassay: The target analytes, three drugs, carbamazepine, diclofenac and caffeine and the f ecal marker isolithocholic acid are covalently coupled to amino-groups on the surface via NHS-chemistry. A competitive immunoassay is subsequently conducted in a simple mix-and-read procedure. Finally, we could use SAFIA to assess the influent of treated and untreated waste water on the Douro river estuary in Portugal. The results of the analysis are comparable to ELISA. However, measurements could be carried out in 25% the time of analysis.zeige mehrzeige weniger

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Metadaten
Autor*innen:Peter Carl
Koautor*innen:Dominik Sarma, I. I. Ramos, A. Machado, Andreas Lehmann, Kristin Hoffmann, Knut Rurack, M. A. Segundo, A. A. Bordalo, Rudolf Schneider
Dokumenttyp:Posterpräsentation
Veröffentlichungsform:Präsentation
Sprache:Englisch
Jahr der Erstveröffentlichung:2017
DDC-Klassifikation:Naturwissenschaften und Mathematik / Chemie / Analytische Chemie
Freie Schlagwörter:Anthropogenic markers; Bead-based assay; Flowcytometry; Immunoassay; Wastewater
Veranstaltung:Adlershofer For­schungs­forum 2017
Veranstaltungsort:Berlin, Germany
Beginndatum der Veranstaltung:10.11.2016
Enddatum der Veranstaltung:10.11.2016
Verfügbarkeit des Dokuments:Datei im Netzwerk der BAM verfügbar ("Closed Access")
Datum der Freischaltung:13.11.2017
Referierte Publikation:Nein
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