Characterization and differentiation of wood rotting fungi by protein and enzyme patterns
- Standardized tests for wood preservatives are performed with defined fungal strains to ensure comparability between laboratories. However, changes of virulence and variation of results are well known events. Suitable and reliable measures to control the stability of the test organisms are necessary.Comparison of protein patterns produced by SDS-electrophoresis was already described by several authors as a possible way to identify and to characterize fungal species and strains, i.e. for Serpula, Coniophora and Poria. We compared protein patterns of several strains of Antrodia vaillantii, Poria placenta, Gloeophyllum trabeum, Lentinus lepideus, and Coniophora puteana. Isoelectric focussing and detection of esterase isoenzymes proved to be an alternative method. The investigations resulted in species-specific protein and enzyme patterns. By comparing the strains of single species it was possible to form groups with similar patterns. Possible misidentifications could be detected. TheStandardized tests for wood preservatives are performed with defined fungal strains to ensure comparability between laboratories. However, changes of virulence and variation of results are well known events. Suitable and reliable measures to control the stability of the test organisms are necessary.Comparison of protein patterns produced by SDS-electrophoresis was already described by several authors as a possible way to identify and to characterize fungal species and strains, i.e. for Serpula, Coniophora and Poria. We compared protein patterns of several strains of Antrodia vaillantii, Poria placenta, Gloeophyllum trabeum, Lentinus lepideus, and Coniophora puteana. Isoelectric focussing and detection of esterase isoenzymes proved to be an alternative method. The investigations resulted in species-specific protein and enzyme patterns. By comparing the strains of single species it was possible to form groups with similar patterns. Possible misidentifications could be detected. The described methods will be further developed and used to follow possible changes in our test strains.…
Autor*innen: | Ute Schoknecht |
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Dokumenttyp: | Beitrag zu einem Tagungsband |
Veröffentlichungsform: | Graue Literatur |
Sprache: | Englisch |
Titel des übergeordneten Werkes (Englisch): | Proceedings of the 30th annual meeting (IRG-WP 99-10298/10326 [u.a.]) |
Jahr der Erstveröffentlichung: | 1999 |
Herausgeber (Institution): | International Research Group on Wood Preservation |
Verlag: | IRG Secretariat |
Verlagsort: | Stockholm |
Ausgabe/Heft: | IRG-WP 99-20177, Section 2 |
Erste Seite: | 1 |
Letzte Seite: | 9(?) |
Freie Schlagwörter: | Basidiomycetes; Enzymes; Isoelectric focussing; Proteins; SDS Polyacrylamide Gel Electrophoresis; Standardization; Strains |
Veranstaltung: | 30th Annual meeting of the International Research Group on Wood Preservation (IRG) |
Veranstaltungsort: | Rosenheim, Germany |
Beginndatum der Veranstaltung: | 1999-06-06 |
Enddatum der Veranstaltung: | 1999-06-11 |
Verfügbarkeit des Dokuments: | Physisches Exemplar in der Bibliothek der BAM vorhanden ("Hardcopy Access") |
Bibliotheksstandort: | Sonderstandort: Publica-Schrank |
Datum der Freischaltung: | 19.02.2016 |
Referierte Publikation: | Nein |