Development of an affinity-based method for the site-selective synthesis of antibody-drug-conjugates
- For the site-selective synthesis of ADCs, a variety of obstacles must be overcome. Those include designing bifunctional affinity peptides with reasonably low 𝐾𝑑-values that couple to the mAb in a site-selective manner. These peptides should also include a functional group that links the payload to the mAb under mild conditions without adversely affecting it. The bioconjugation between peptide and antibody and the linker between peptide and payload must be stable and durable to provide safety when used for medical purposes. The usage of metals and organic solvents should be minimized. Within the project, new types of functionalized affinity peptides were designed, and their affinity towards the Fc-fragment was determined.
Autor*innen: | Mariella Gabler |
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Dokumenttyp: | Sonstiges |
Veröffentlichungsform: | Graue Literatur |
Sprache: | Englisch |
Titel des übergeordneten Werkes (Englisch): | Masterarbeit |
Jahr der Erstveröffentlichung: | 2021 |
Organisationseinheit der BAM: | 1 Analytische Chemie; Referenzmaterialien |
1 Analytische Chemie; Referenzmaterialien / 1.5 Proteinanalytik | |
Gutachter*innen: | Michael G. WellerORCiD, M. Linscheid |
Verlag: | Humboldt-Universität zu Berlin |
Verlagsort: | Berlin |
Erste Seite: | 1 |
Letzte Seite: | 100 |
DDC-Klassifikation: | Technik, Medizin, angewandte Wissenschaften / Ingenieurwissenschaften / Ingenieurwissenschaften und zugeordnete Tätigkeiten |
Freie Schlagwörter: | ADC; Affinity; Antibody drug conjugate; Click chemistry; Copper-catalyzed; DM1; HPLC; Herceptin; Human antibody; Linker; MALDI-TOF-MS; MMAE; Mertansine; Monomethyl Auristatin E; Payload; Peptide; SDS-PAGE; SPR; Site-selective bioconjugation; Toxin; Trastuzumab |
Themenfelder/Aktivitätsfelder der BAM: | Material |
Material / Materialdesign | |
Verfügbarkeit des Dokuments: | Datei im Netzwerk der BAM verfügbar ("Closed Access") |
Datum der Freischaltung: | 23.03.2022 |
Referierte Publikation: | Nein |