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Macrophage-uptake of sialic acid-targeted molecularly imprinted polymers (SA-MIPs)

  • Sialic acid (SA) is a cell surface glycan, which has a decisive role in many cell activities including differentiation, proliferation, and the immune response. The amount of SA has been found to correlate with cancer, with an upregulation on more aggressive cancers. Therefore, there is a great interest in developing methods for detection of SA on cancer cells. We are screening SA on cancer cell lines by using fluorescent molecularly imprinted polymers, SA-MIPs. Macrophages, which evolve from mono-cytes, are well known for their extraordinary ability to phagocytose foreign objects. This could lead to the hypothesis that the SA-MIPs can be recognized by macrophages as foreign object; thus leading to internalization and potentially degradation. We have demonstrated that SA-MIPs can be detected after incubation with the RAW macrophage cells, with increasing fluorescence over time. The microscopy analysis shows that the RAW cells ingest the SA-MIP particles. This information is importantSialic acid (SA) is a cell surface glycan, which has a decisive role in many cell activities including differentiation, proliferation, and the immune response. The amount of SA has been found to correlate with cancer, with an upregulation on more aggressive cancers. Therefore, there is a great interest in developing methods for detection of SA on cancer cells. We are screening SA on cancer cell lines by using fluorescent molecularly imprinted polymers, SA-MIPs. Macrophages, which evolve from mono-cytes, are well known for their extraordinary ability to phagocytose foreign objects. This could lead to the hypothesis that the SA-MIPs can be recognized by macrophages as foreign object; thus leading to internalization and potentially degradation. We have demonstrated that SA-MIPs can be detected after incubation with the RAW macrophage cells, with increasing fluorescence over time. The microscopy analysis shows that the RAW cells ingest the SA-MIP particles. This information is important when planning to use SA-MIPs in future in vivo applications.zeige mehrzeige weniger

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Metadaten
Autor*innen:L. Sternbaek
Koautor*innen:Martha Wamaitha Kimani, S. Shinde, K. Alm, B. Sellegren, A. Gjörloff-Wingren
Dokumenttyp:Posterpräsentation
Veröffentlichungsform:Präsentation
Sprache:Englisch
Jahr der Erstveröffentlichung:2018
Organisationseinheit der BAM:1 Analytische Chemie; Referenzmaterialien
1 Analytische Chemie; Referenzmaterialien / 1.9 Chemische und optische Sensorik
DDC-Klassifikation:Naturwissenschaften und Mathematik / Chemie / Analytische Chemie
Technik, Medizin, angewandte Wissenschaften / Ingenieurwissenschaften / Ingenieurwissenschaften und zugeordnete Tätigkeiten
Freie Schlagwörter:MIPs; Macrophages; Sialic acid
Themenfelder/Aktivitätsfelder der BAM:Material
Umwelt
Umwelt / Sensorik
Veranstaltung:1st National Meeting of the Swedish Chemical Society
Veranstaltungsort:Lund University, Sweden
Beginndatum der Veranstaltung:17.06.2018
Enddatum der Veranstaltung:20.06.2018
Verfügbarkeit des Dokuments:Datei im Netzwerk der BAM verfügbar ("Closed Access")
Datum der Freischaltung:10.07.2018
Referierte Publikation:Nein
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