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Colloidal metalorganic frameworks as novel biofunctional nanoparticles for immunoassay applications

  • Metal-organic framework (MOF) colloids have unique features that render them ideal signalling agents for realizing advanced immunoassay-based detection systems. MOFs are porous coordination polymers of metal nodes and organic linkers. The pore size of MOFs can be engineered and tailored to allow specific host (MOF) and guest (analyte) interactions. The particle sizes of the colloidal MOF can be tailored by employing methods from colloidal chemistry in wet synthesis. The adaption of established Layer-by-Layer polyelectrolyte coating protocols [1] allows equipping colloidal MOF particles with a nanometer thin polyelectrolyte membrane. This polyelectrolyte membrane serves as an interface for antibody binding. These biofunctional MOF nanoparticles have shown a strong immuno-binding that is sufficient for solid state immunoassays. Our current research addresses the design of luminescence encoded colloidal particle libraries by adjusting the ratios of e.g. Terbium (green) and Europium (red)Metal-organic framework (MOF) colloids have unique features that render them ideal signalling agents for realizing advanced immunoassay-based detection systems. MOFs are porous coordination polymers of metal nodes and organic linkers. The pore size of MOFs can be engineered and tailored to allow specific host (MOF) and guest (analyte) interactions. The particle sizes of the colloidal MOF can be tailored by employing methods from colloidal chemistry in wet synthesis. The adaption of established Layer-by-Layer polyelectrolyte coating protocols [1] allows equipping colloidal MOF particles with a nanometer thin polyelectrolyte membrane. This polyelectrolyte membrane serves as an interface for antibody binding. These biofunctional MOF nanoparticles have shown a strong immuno-binding that is sufficient for solid state immunoassays. Our current research addresses the design of luminescence encoded colloidal particle libraries by adjusting the ratios of e.g. Terbium (green) and Europium (red) metal nodes in mixed lanthanide based MOF-76. These mixed lanthanide MOF-76 particles are envisioned to allow multiplexed immuno-detection of endocrine disruptors such as bisphenol A. In addition we investigate the detection of analytes that do not allow the production of antibodies due to their inherent properties. Such “difficult analytes” have a strong hydrophobicity or are very small or highly toxic molecules. One example is the common plasticizer dioctylphthalate that is also a potent endocrine disruptor. MOF colloids can address this issue by specific host (MOF) : guest (analyte) interactions that result in analyte-specific colour change or exciplex-based fluorescence emission. Our overall aim is to develop methodologies that allow parallel sensing of two endocrine disruptors (e.g. bisphenol A & phthalates) by simultaneous immuno-detection and MOF:analyte specific interactions.zeige mehrzeige weniger

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Metadaten
Autor*innen:Sebastian Beyer
Koautor*innen:Kevin Rothfahl, Ander Chapartegui, Franziska Emmerling, Rudolf Schneider
Dokumenttyp:Vortrag
Veröffentlichungsform:Präsentation
Sprache:Englisch
Jahr der Erstveröffentlichung:2017
DDC-Klassifikation:Naturwissenschaften und Mathematik / Chemie / Analytische Chemie
Freie Schlagwörter:Immunoassay; MOFs; Nanoparticles
Veranstaltung:BioSensor 2017 - 1st European and 10th German BioSensor Symposium
Veranstaltungsort:Potsdam, Germany
Beginndatum der Veranstaltung:20.03.2017
Enddatum der Veranstaltung:23.03.2017
Verfügbarkeit des Dokuments:Datei im Netzwerk der BAM verfügbar ("Closed Access")
Datum der Freischaltung:02.01.2018
Referierte Publikation:Nein
Eingeladener Vortrag:Nein
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