Reference Materials for Standardization of Fluorescence-based Measurements
- Here, we summerize our efforts concerning new design concepts and examples for fluorescence standards that can provide traceability to radiometric units and present a first step towards a toolbox of fluorescence standards, currently consisting of: i) A first set of liquid fluorescence standards enables the determination of a broad variety of fluorescence parameters was developed and certified by BAM and is distributed by Sigma-Aldrich. ii) Ready-to-use, glass-based fluorescence standards for instrument performance validation (IPV) and determination instrument-to-instrument variations can also be used as wavelength standard for fluorescence instruments with low requirements on spectral resolution and allow monitoring of temporal changes of the wavelength-dependent spectral responsivity. iii) Novel calibration tools and validation concepts for microarray-based platforms used in molecular diagnostics and food safety control. iv) We currently develop reference materials, which can beHere, we summerize our efforts concerning new design concepts and examples for fluorescence standards that can provide traceability to radiometric units and present a first step towards a toolbox of fluorescence standards, currently consisting of: i) A first set of liquid fluorescence standards enables the determination of a broad variety of fluorescence parameters was developed and certified by BAM and is distributed by Sigma-Aldrich. ii) Ready-to-use, glass-based fluorescence standards for instrument performance validation (IPV) and determination instrument-to-instrument variations can also be used as wavelength standard for fluorescence instruments with low requirements on spectral resolution and allow monitoring of temporal changes of the wavelength-dependent spectral responsivity. iii) Novel calibration tools and validation concepts for microarray-based platforms used in molecular diagnostics and food safety control. iv) We currently develop reference materials, which can be used as reliable quantum yield standards for relative methods for the determination of QY and can be valuable in the evaluation of the performance and sources of uncertainty of absolute, standard-free methods using e.g. integrating spheres.…
Autor*innen: | Katrin HoffmannORCiD |
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Koautor*innen: | Angelika Hoffmann, Jutta Pauli, Monika Spieles, Claudia Brunner, Ute Resch-GengerORCiD |
Dokumenttyp: | Posterpräsentation |
Veröffentlichungsform: | Präsentation |
Sprache: | Englisch |
Jahr der Erstveröffentlichung: | 2015 |
DDC-Klassifikation: | Naturwissenschaften und Mathematik / Chemie / Analytische Chemie |
Freie Schlagwörter: | Fluorescence; Microarray; Quantum yield; Standards |
Veranstaltung: | 14th Conference on Methods and Applications in Fluorescence |
Veranstaltungsort: | Würzburg, Germany |
Beginndatum der Veranstaltung: | 13.09.2015 |
Enddatum der Veranstaltung: | 16.09.2015 |
Verfügbarkeit des Dokuments: | Datei im Netzwerk der BAM verfügbar ("Closed Access") |
Datum der Freischaltung: | 04.01.2017 |
Referierte Publikation: | Nein |