Ectoine protects biomolecules from ionizing radiation: Molecular mechanisms
- The compatible solute and osmolyte ectoine is an effective protectant of biomolecules and whole cells against heating, freezing and high salinity. The protection of cells (human Keratinocytes) by ectoine against ultraviolet radiation was also reported by various authors, although the underlying mechanism is not yet understood. We present results on the irradiation of biomolecules (DNA) with ionizing radiation (high energy electrons) in fully aqueous environment in the presence of ectoine and high salt concentrations. The results demonstrate an effective radiation protection of DNA by ectoine against the induction of single strand breaks by ionizing radiation. The effect is explained by an increased in low-energy electron scattering at the enhanced free-vibrational density of states of water due to ectoine, as well as the action of ectoine as an OH-radical scavenger. This was demonstrated by Raman spectroscopy, electron paramagnetic resonance (EPR) and Monte-Carlo simulations (Geant4).
Autor*innen: | Marc Benjamin HahnORCiD |
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Koautor*innen: | S. Meyer, M.-A. Schröter, Hans-Jörg Kunte, Tihomir Solomun, Heinz Sturm |
Dokumenttyp: | Posterpräsentation |
Veröffentlichungsform: | Präsentation |
Sprache: | Englisch |
Jahr der Erstveröffentlichung: | 2023 |
Organisationseinheit der BAM: | 6 Materialchemie |
6 Materialchemie / 6.6 Physik und chemische Analytik der Polymere | |
DDC-Klassifikation: | Naturwissenschaften und Mathematik / Chemie / Analytische Chemie |
Freie Schlagwörter: | Compatible solute; DNA; Dosimetry; Ectoin; Ectoine; Ectoine radiation protection; Ectoine radical scavenger; Geant4; Geant4-DNA; Hydroxyectoine; Hydroxyl radical; Ionizing radiation; LEE; Low energy electrons; OH-radical; Osmolyte; Radiation damage; Radiation protection; Radiation therapy; Radical scavenger |
Themenfelder/Aktivitätsfelder der BAM: | Chemie und Prozesstechnik |
Veranstaltung: | #RSCposter 2023 |
Veranstaltungsort: | Online meeting |
Beginndatum der Veranstaltung: | 28.02.2023 |
Enddatum der Veranstaltung: | 01.03.2023 |
Verfügbarkeit des Dokuments: | Datei im Netzwerk der BAM verfügbar ("Closed Access") |
Datum der Freischaltung: | 02.03.2023 |
Referierte Publikation: | Nein |