Simple and versatile methods for quantifying functional groups, ligands, and biomolecules on nanomaterials
- Many applications of nanomaterials in the life sciences require the controlled functionalization of these materials with ligands like polyethylene glycol (PEG) and/or biomolecules such as peptides, proteins, and DNA. This enables to tune their hydrophilicity and biocompatibility, minimize unspecific interactions, improve biofunction-nalization efficiencies, and enhance blood circulation times and is the ultimate prerequisite for their use as reporters in assays or the design of targeted optial probes for bioimaging. At the core of these functionalization strategies are reliable and validated methods for surface group and ligand quantification that can be preferably performed with routine laboratory instrumentation, require only small amounts of substances, and are suitable for many different types of nanomaterials. We present here versatile and simple concepts for the quantification of common functional groups, ligands, and biomolecules on different types of organic and inorganicMany applications of nanomaterials in the life sciences require the controlled functionalization of these materials with ligands like polyethylene glycol (PEG) and/or biomolecules such as peptides, proteins, and DNA. This enables to tune their hydrophilicity and biocompatibility, minimize unspecific interactions, improve biofunction-nalization efficiencies, and enhance blood circulation times and is the ultimate prerequisite for their use as reporters in assays or the design of targeted optial probes for bioimaging. At the core of these functionalization strategies are reliable and validated methods for surface group and ligand quantification that can be preferably performed with routine laboratory instrumentation, require only small amounts of substances, and are suitable for many different types of nanomaterials. We present here versatile and simple concepts for the quantification of common functional groups, ligands, and biomolecules on different types of organic and inorganic nano-materials, using different types of optical reporters and method validation with the aid of multimodal reporters and mass balances.…
Autor*innen: | Ute Resch-GengerORCiD |
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Koautor*innen: | Nithiya Nirmalananthan-Budau, Marko Moser, Daniel Geißsler, Thomas Behnke, Ralf Schneider |
Dokumenttyp: | Vortrag |
Veröffentlichungsform: | Präsentation |
Sprache: | Englisch |
Jahr der Erstveröffentlichung: | 2018 |
Organisationseinheit der BAM: | 1 Analytische Chemie; Referenzmaterialien |
1 Analytische Chemie; Referenzmaterialien / 1.2 Biophotonik | |
DDC-Klassifikation: | Naturwissenschaften und Mathematik / Chemie / Analytische Chemie |
Technik, Medizin, angewandte Wissenschaften / Ingenieurwissenschaften / Ingenieurwissenschaften und zugeordnete Tätigkeiten | |
Freie Schlagwörter: | Absolute fluorescence quantum yield; Fluorescence decay kinetics; Integrating sphere spectroscopy; PEG ligands; Surface group analysis; Upconverting nanoparticles |
Themenfelder/Aktivitätsfelder der BAM: | Chemie und Prozesstechnik |
Material | |
Material / Degradation von Werkstoffen | |
Umwelt | |
Umwelt / Sensorik | |
Veranstaltung: | RSC Symposium on Nanoparticle concentration – critical needs and state-of-the-art measurement |
Veranstaltungsort: | London, UK |
Beginndatum der Veranstaltung: | 24.04.2018 |
Enddatum der Veranstaltung: | 25.04.2018 |
Bemerkung: | Geburtsname von Nirmalananthan-Budau, Nithiya: Nirmalananthan, N. - Birth name of Nirmalananthan-Budau, Nithiya: Nirmalananthan, N. |
Verfügbarkeit des Dokuments: | Datei im Netzwerk der BAM verfügbar ("Closed Access") |
Datum der Freischaltung: | 23.05.2018 |
Referierte Publikation: | Nein |
Eingeladener Vortrag: | Nein |