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Upconverting Nanoparticle-based Enhanced Luminescence Lateral-Flow Assay for Urinary Biomarker Monitoring

  • Development of efficient portable sensors for accurately detecting biomarkers is crucial for early disease diagnosis, yet remains a significant challenge. To address this need, we introduce the enhanced luminescence lateral-flow assay, which leverages highly luminescent upconverting nanoparticles (UCNPs) alongside a portable reader and a smartphone app. The sensor’s efficiency and versatility were shown for kidney health monitoring as a proof of concept. We engineered Er3+- and Tm3+-doped UCNPs coated with multiple layers, including an undoped inert matrix shell, a mesoporous silica shell, and an outer layer of gold (UCNP@mSiO2@Au). These coatings synergistically enhance emission by over 40-fold and facilitate biomolecule conjugation, rendering UCNP@mSiO2@Au easy to use and suitable for a broad range of bioapplications. Employing these optimized nanoparticles in lateral-flow assays, we successfully detected two acute kidney injury-related biomarkers-kidney injury molecule-1 (KIM-1)Development of efficient portable sensors for accurately detecting biomarkers is crucial for early disease diagnosis, yet remains a significant challenge. To address this need, we introduce the enhanced luminescence lateral-flow assay, which leverages highly luminescent upconverting nanoparticles (UCNPs) alongside a portable reader and a smartphone app. The sensor’s efficiency and versatility were shown for kidney health monitoring as a proof of concept. We engineered Er3+- and Tm3+-doped UCNPs coated with multiple layers, including an undoped inert matrix shell, a mesoporous silica shell, and an outer layer of gold (UCNP@mSiO2@Au). These coatings synergistically enhance emission by over 40-fold and facilitate biomolecule conjugation, rendering UCNP@mSiO2@Au easy to use and suitable for a broad range of bioapplications. Employing these optimized nanoparticles in lateral-flow assays, we successfully detected two acute kidney injury-related biomarkers-kidney injury molecule-1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL)-in urine samples. Using our sensor platform, KIM-1 and NGAL can be accurately detected and quantified within the range of 0.1 to 20 ng/mL, boasting impressively low limits of detection at 0.28 and 0.23 ng/mL, respectively. Validating our approach, we analyzed clinical urine samples, achieving biomarker concentrations that closely correlated with results obtained via ELISA. Importantly, our system enables biomarker quantification in less than 15 min, underscoring the performance of our novel UCNP-based approach and its potential as reliable, rapid, and user-friendly diagnostics.zeige mehrzeige weniger

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Autor*innen:Marylyn Setsuko AraiORCiD, Hyunho KimORCiD, Madeleine Pascavis, Baekdong ChaORCiD, Gabriel BrambillaORCiD, Young Kwan ChoORCiD, Jinho ParkORCiD, Raquel R. C. VilelaORCiD, Andrea S. S. de CamargoORCiD, Cesar M. CastroORCiD, Hakho LeeORCiD
Dokumenttyp:Zeitschriftenartikel
Veröffentlichungsform:Verlagsliteratur
Sprache:Englisch
Titel des übergeordneten Werkes (Englisch):ACS Applied Materials & Interfaces
Jahr der Erstveröffentlichung:2024
Organisationseinheit der BAM:5 Werkstofftechnik
5 Werkstofftechnik / 5.6 Glas
Verlag:American Chemical Society (ACS)
Jahrgang/Band:16
Ausgabe/Heft:29
Erste Seite:38243
Letzte Seite:38251
DDC-Klassifikation:Technik, Medizin, angewandte Wissenschaften / Ingenieurwissenschaften / Ingenieurwissenschaften und zugeordnete Tätigkeiten
Freie Schlagwörter:Biosensor; Kidney injury; Lateral flow; Portable sensor; Upconverting nanoparticles
Themenfelder/Aktivitätsfelder der BAM:Material
Material / Materialdesign
Material / Nano
DOI:10.1021/acsami.4c06117
Verfügbarkeit des Dokuments:Datei im Netzwerk der BAM verfügbar ("Closed Access")
Datum der Freischaltung:25.07.2024
Referierte Publikation:Ja
Datum der Eintragung als referierte Publikation:25.07.2024
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