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Development of a quick test to screen for bisphenol A release from polymer materials

  • Bisphenol A (BPA) is a common industrial chemical that is widely used as a monomer or additive in the production of polycarbonate plastics and other polymeric materials. The primary way of exposure to BPA for most people is assumed to be a direct con-tamination of food and water through use of containers, bottles, and other plastic stor-age. BPA has been identified as an important endocrine disrupting compound (EDC). It can interfere with hormonal activities like biosynthesis, metabolism and elimination of natural blood-borne hormones. Moreover, BPA can originate environmental problems. A spectrum of methods have been developed with the aim of determining BPA in water. Immunoassays appear to comply with sensitivity, rapidity, and low cost requirements for effective monitoring. In this work a comparative analysis was conducted of the effect of temperature and the choice of buffers, as well as the substrate at various stages of an ELISA (enzyme-linked immunosorbent assay). The methodBisphenol A (BPA) is a common industrial chemical that is widely used as a monomer or additive in the production of polycarbonate plastics and other polymeric materials. The primary way of exposure to BPA for most people is assumed to be a direct con-tamination of food and water through use of containers, bottles, and other plastic stor-age. BPA has been identified as an important endocrine disrupting compound (EDC). It can interfere with hormonal activities like biosynthesis, metabolism and elimination of natural blood-borne hormones. Moreover, BPA can originate environmental problems. A spectrum of methods have been developed with the aim of determining BPA in water. Immunoassays appear to comply with sensitivity, rapidity, and low cost requirements for effective monitoring. In this work a comparative analysis was conducted of the effect of temperature and the choice of buffers, as well as the substrate at various stages of an ELISA (enzyme-linked immunosorbent assay). The method has been optimized and has shown good repeatability and sensitivity. The results obtained from ELISA will be used as the basis for the development of a Lateral Flow ImmunoAssay (LFIA).zeige mehrzeige weniger

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Metadaten
Autoren/innen:Anna Raysyan
Koautoren/innen:Bianca Coesfeld, Rudolf J. Schneider
Dokumenttyp:Posterpräsentation
Veröffentlichungsform:Präsentation
Sprache:Englisch
Jahr der Erstveröffentlichung:2017
Organisationseinheit der BAM:1 Analytische Chemie; Referenzmaterialien
1 Analytische Chemie; Referenzmaterialien / 1.8 Umweltanalytik
DDC-Klassifikation:Technik, Medizin, angewandte Wissenschaften / Ingenieurwissenschaften / Sanitär- und Kommunaltechnik; Umwelttechnik
Freie Schlagwörter:Bisphenol A; ELISA; Immunoassay
Themenfelder/Aktivitätsfelder der BAM:Umwelt
Umwelt / Umweltschadstoffe
Veranstaltung:EBS 2017
Veranstaltungsort:Potsdam, Germany
Beginndatum der Veranstaltung:20.03.2017
Enddatum der Veranstaltung:23.03.2017
Verfügbarkeit des Dokuments:Datei im Netzwerk der BAM verfügbar ("Closed Access")
Datum der Freischaltung:12.04.2017
Referierte Publikation:Nein