V.G. Bandi, M.P. Luciano, M. Saccomano, N.L. Patel, Th. S. Bischof, J.G.P. Lingg, P.T. Tsrunchev, M.N. Nix, Bastian Ruehle, C. Sanders, L. Riffle, C.M. Robinson, S. Difilippantonio, J.D. Kalen, Ute Resch-Genger, J. Ivanic, O.T. Bruns, M. Schnermann
- Recent progress has shown that using wavelengths between 1,000 and 2,000 nm, referred to as the shortwave-infrared or near-infrared (NIR)-II range, can enable high-resolution in vivo imaging at depths not possible with conventional optical wavelengths.
However, few bioconjugatable probes of the type that have proven invaluable for multiplexed imaging in the visible and NIR range are available for imaging these wavelengths. Using rational design, we have generated persulfonated indocyanine dyes with absorbance maxima at 872 and 1,072 nm through catechol-ring and aryl-ring fusion, respectively, onto the nonamethine scaffold. Multiplexed two-color and three-color in vivo imaging using monoclonal antibody and dextran conjugates in several tumor models illustrate the benefits of concurrent labeling of the tumor and healthy surrounding tissue and lymphatics.
These efforts are enabled by complementary advances in a custom-built NIR/shortwave-infrared imaging setup and software package forRecent progress has shown that using wavelengths between 1,000 and 2,000 nm, referred to as the shortwave-infrared or near-infrared (NIR)-II range, can enable high-resolution in vivo imaging at depths not possible with conventional optical wavelengths.
However, few bioconjugatable probes of the type that have proven invaluable for multiplexed imaging in the visible and NIR range are available for imaging these wavelengths. Using rational design, we have generated persulfonated indocyanine dyes with absorbance maxima at 872 and 1,072 nm through catechol-ring and aryl-ring fusion, respectively, onto the nonamethine scaffold. Multiplexed two-color and three-color in vivo imaging using monoclonal antibody and dextran conjugates in several tumor models illustrate the benefits of concurrent labeling of the tumor and healthy surrounding tissue and lymphatics.
These efforts are enabled by complementary advances in a custom-built NIR/shortwave-infrared imaging setup and software package for multicolor real-time imaging.…
MetadatenAutor*innen: | V.G. Bandi, M.P. Luciano, M. Saccomano, N.L. Patel, Th. S. Bischof, J.G.P. Lingg, P.T. Tsrunchev, M.N. Nix, Bastian Ruehle, C. Sanders, L. Riffle, C.M. Robinson, S. Difilippantonio, J.D. Kalen, Ute Resch-GengerORCiD, J. Ivanic, O.T. Bruns, M. Schnermann |
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Dokumenttyp: | Zeitschriftenartikel |
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Veröffentlichungsform: | Verlagsliteratur |
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Sprache: | Englisch |
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Titel des übergeordneten Werkes (Englisch): | Nature Methods |
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Jahr der Erstveröffentlichung: | 2021 |
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Organisationseinheit der BAM: | 1 Analytische Chemie; Referenzmaterialien |
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| 1 Analytische Chemie; Referenzmaterialien / 1.2 Biophotonik |
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Verlag: | Nature Research |
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Jahrgang/Band: | 19 |
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Ausgabe/Heft: | 3 |
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Erste Seite: | 353 |
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Letzte Seite: | 358 |
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DDC-Klassifikation: | Naturwissenschaften und Mathematik / Chemie / Analytische Chemie |
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| Technik, Medizin, angewandte Wissenschaften / Ingenieurwissenschaften / Ingenieurwissenschaften und zugeordnete Tätigkeiten |
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Freie Schlagwörter: | Antibody; Application; Bioconjugate; Bioimaging; Conjugate; Contrast agent; Cyanine; Dye; Fluorescence; Imaging; Lifetime; Mechanism; NIR; Photoluminescence; Photophysics; Quantum yield; SWIR; Sensor |
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Themenfelder/Aktivitätsfelder der BAM: | Chemie und Prozesstechnik |
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| Material |
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| Umwelt |
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| Umwelt / Sensorik |
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DOI: | 10.1038/s41592-022-01394-6 |
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Verfügbarkeit des Dokuments: | Datei im Netzwerk der BAM verfügbar ("Closed Access") |
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Datum der Freischaltung: | 14.03.2022 |
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Referierte Publikation: | Ja |
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Datum der Eintragung als referierte Publikation: | 14.03.2022 |
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