Multifunctional nanocarriers for biomedical applications
- Polymeric vesicles (Pluronic® L-121) loaded with magnetic nanoparticles (MNP) and an anti-cancer drug (camptothecin) were prepared continuously in a micro mixing device. Characterization by TEM confirmed the successful incorporation of the MNP and DLS measurements showed a relatively narrow size distribution of the hybrid polymersomes. A very high drug loading of camptothecin (100 µg/ml in the polymersome formulation) was reached and a drug release study of loaded magnetic polymersomes has shown a sustained camptothecin release over several days. Carboxylation of Pluronic® L-121 was performed and enabled a further surface functionalization with bombesin, a 14 amino acid peptide, which binds specifically to the GRPR (gastrin releasing peptide receptor). This receptor is often overexpressed in tumor cells (e.g., human prostate cancer cells) and therefore a suitable target for cancer treatment. An additional fluorescence label with Alexa Fluor® 647 allow tracking of the polymersomes e.g.,Polymeric vesicles (Pluronic® L-121) loaded with magnetic nanoparticles (MNP) and an anti-cancer drug (camptothecin) were prepared continuously in a micro mixing device. Characterization by TEM confirmed the successful incorporation of the MNP and DLS measurements showed a relatively narrow size distribution of the hybrid polymersomes. A very high drug loading of camptothecin (100 µg/ml in the polymersome formulation) was reached and a drug release study of loaded magnetic polymersomes has shown a sustained camptothecin release over several days. Carboxylation of Pluronic® L-121 was performed and enabled a further surface functionalization with bombesin, a 14 amino acid peptide, which binds specifically to the GRPR (gastrin releasing peptide receptor). This receptor is often overexpressed in tumor cells (e.g., human prostate cancer cells) and therefore a suitable target for cancer treatment. An additional fluorescence label with Alexa Fluor® 647 allow tracking of the polymersomes e.g., in cell experiments. Relaxivity measurements to evaluate the potential of magnetic polymersomes as MR contrast agent for in vivo imaging are in progress.…
Autor*innen: | Regina Bleul, Raphael Thiermann, K. Saatchi, U.O. Häfeli, M. Maskos |
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Persönliche Herausgeber*innen: | W.J. Parak, M. Osinski, K. Yamamoto |
Dokumenttyp: | Beitrag zu einem Tagungsband |
Veröffentlichungsform: | Verlagsliteratur |
Sprache: | Englisch |
Titel des übergeordneten Werkes (Englisch): | Colloidal nanocrystals for biomedical applications VIII (Proceedings of SPIE) |
Jahr der Erstveröffentlichung: | 2013 |
Herausgeber (Institution): | Society of photo-optical instrumentation engineers (SPIE) |
Jahrgang/Band: | 8595 |
Erste Seite: | 85951N-1 - 85951N-9 |
Freie Schlagwörter: | Block copolymers; Drug delivery; Micro mixer; Pluronics; Polymersomes; Vesicles |
Veranstaltung: | Colloidal nanocrystals for biomedical applications VIII |
Veranstaltungsort: | San Francisco, CA, USA |
Beginndatum der Veranstaltung: | 2013-02-22 |
Enddatum der Veranstaltung: | 2013-02-22 |
DOI: | 10.1117/12.2008194 |
ISBN: | 9780819493644 |
Bemerkung: | Serientitel: Proceedings of SPIE – Series title: Proceedings of SPIE |
Verfügbarkeit des Dokuments: | Physisches Exemplar in der Bibliothek der BAM vorhanden ("Hardcopy Access") |
Bibliotheksstandort: | Sonderstandort: Publica-Schrank |
Datum der Freischaltung: | 20.02.2016 |
Referierte Publikation: | Ja |
Datum der Eintragung als referierte Publikation: | 23.05.2013 |