Initial attachment of bacteria on PE-HD by fluorescence microscopy and colony-forming unit
- The first documentation of fuel biodeterioration dates back to the late 19th century. However, extensive studies concerning the microbial fuel contamination started in 1980’s. Polymeric fuel storage tanks containing diesel and biodiesel provide environmental conditions for microbial growth. Several studies demonstrated that bacteria, which were found in contaminated fuel systems, can use fuels as macronutrient; but such bacteria can also cause microbiologically influenced corrosion and fouling.
The aim of this study is to investigate the initial attachment behavior of bacteria, isolated from a diesel contamination, on neat and photooxidized high-density polyethylene (PE-HD). Two common PE-HD’s, less- and biodiesel-stabilized, were radiated to UV light representing a tank exposed to sunlight. The effect of photooxidiation on PE-HD’s surface were characterized chemically by Fourier-transform infrared spectroscopy (FTIR). The attached bacteria Pseudomonas aeruginosa and BacillusThe first documentation of fuel biodeterioration dates back to the late 19th century. However, extensive studies concerning the microbial fuel contamination started in 1980’s. Polymeric fuel storage tanks containing diesel and biodiesel provide environmental conditions for microbial growth. Several studies demonstrated that bacteria, which were found in contaminated fuel systems, can use fuels as macronutrient; but such bacteria can also cause microbiologically influenced corrosion and fouling.
The aim of this study is to investigate the initial attachment behavior of bacteria, isolated from a diesel contamination, on neat and photooxidized high-density polyethylene (PE-HD). Two common PE-HD’s, less- and biodiesel-stabilized, were radiated to UV light representing a tank exposed to sunlight. The effect of photooxidiation on PE-HD’s surface were characterized chemically by Fourier-transform infrared spectroscopy (FTIR). The attached bacteria Pseudomonas aeruginosa and Bacillus subtilis on the polymer surface were evaluated by fluorescence microscopy and colony-forming unit tests (CFU).…
Autor*innen: | Maren Erdmann |
---|---|
Weitere Person: | Sherin Kleinbub, Martin BöhningORCiD, Ute Niebergall, Andrea KoerdtORCiD |
Dokumenttyp: | Vortrag |
Veröffentlichungsform: | Präsentation |
Sprache: | Englisch |
Jahr der Erstveröffentlichung: | 2018 |
Organisationseinheit der BAM: | 4 Material und Umwelt |
4 Material und Umwelt / 4.1 Biologische Materialschädigung und Referenzorganismen | |
5 Werkstofftechnik | |
5 Werkstofftechnik / 5.3 Polymere Verbundwerkstoffe | |
7 Bauwerkssicherheit | |
7 Bauwerkssicherheit / 7.5 Technische Eigenschaften von Polymerwerkstoffen | |
DDC-Klassifikation: | Technik, Medizin, angewandte Wissenschaften / Ingenieurwissenschaften / Ingenieurwissenschaften und zugeordnete Tätigkeiten |
Freie Schlagwörter: | Initial attachment; PE-HD; UV-irradiation |
Themenfelder/Aktivitätsfelder der BAM: | Material |
Material / Degradation von Werkstoffen | |
Veranstaltung: | MoDeSt2018 |
Veranstaltungsort: | Tokio, Japan |
Beginndatum der Veranstaltung: | 02.09.2018 |
Enddatum der Veranstaltung: | 06.09.2018 |
Verfügbarkeit des Dokuments: | Datei im Netzwerk der BAM verfügbar ("Closed Access") |
Datum der Freischaltung: | 11.09.2018 |
Referierte Publikation: | Nein |
Eingeladener Vortrag: | Nein |