Determination and identification of hydrophilic and hydrophobic arsenic species in methanol extract of fresh cod liver by RP-HPLC with simultaneous ICP-MS and ESI-Q-TOF-MS detection
- The present study was focused on the determination and identification of arsenic species in methanolic extracts of cod liver. Arsenic species were fractionated and the fractions analysed by RP-HPLC-ICP-MS coupled with ESI-Q-TOF-MS. The total concentration of arsenic in the fresh cod liver was analysed by ICP-MS to be 1.53 ± 0.02 mg As kg-1 w.w. and the extraction recovery was ca. 100% and the column recovery >93%. Besides polar inorganic and methylated arsenic species (>70%) more hydrophobic arsenic-containing fatty acids and hydrocarbons occurred. Based on the mass spectrometric data proposals for molecular structures were elaborated for 20 of the organic As species included 10 arsenic-containing fatty acids (AsFA) and an arsenic-containing hydrocarbon (AsHC) mentioned for the first time in fresh cod liver. Arsenobetaine was found as main water-soluble arsenic compound in cod liver followed by higher molecular mass arsenic-containing fatty acids and hydrocarbons.
Autor*innen: | Uriel Arroyo-Abad, Susanne Lischka, Christian Piechotta, J. Mattusch, T. Reemtsma |
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Dokumenttyp: | Zeitschriftenartikel |
Veröffentlichungsform: | Verlagsliteratur |
Sprache: | Englisch |
Titel des übergeordneten Werkes (Englisch): | Food chemistry |
Jahr der Erstveröffentlichung: | 2013 |
Verlag: | Elsevier |
Verlagsort: | Amsterdam [u.a.] ; Jena |
Jahrgang/Band: | 141 |
Ausgabe/Heft: | 3 |
Erste Seite: | 3093 |
Letzte Seite: | 3102 |
Freie Schlagwörter: | Arsenic species; Arsenolipids; Cod liver; HPLC-ICP-MS/ESI-Q-TOF-MS; Marine fish |
DOI: | 10.1016/j.foodchem.2013.05.152 |
ISSN: | 0308-8146 |
Verfügbarkeit des Dokuments: | Datei im Netzwerk der BAM verfügbar ("Closed Access") |
Datum der Freischaltung: | 20.02.2016 |
Referierte Publikation: | Ja |
Datum der Eintragung als referierte Publikation: | 01.08.2013 |