• Treffer 1 von 1
Zurück zur Trefferliste

Exploiding termite nest microbiomes for antibiotic drug discovery using an integrated high throughput Microfluidics/FACS pipeline

  • Bacterial infections are a global threat to human health1. Especially nosocomial infections with multidrug-resistant Gram-negative bacteria challenge public health systems and endanger successful treatment of patients in various modern medicine applications as e.g. organ transplant. In the last three decades classical approaches failed to find novel chemical scaffolds suitable for the development of antibiotics. Insect microbiomes produce potent antibiotics with low toxicity to eukaryotic cells to defend their hosts against entomopathogenic microorganisms2. In order to exploit insect microbiomes efficiently and to identify novel antibiotic producing bacteria, we implemented an high throughput Microfluidics/FACS (fluorescence-associated cell sorting) cultivation and screening pipeline. Here, we present the technical aspects of our pipeline including downstream processing. This includes genomic fingerprinting of identified producer strains, metabolomic analysis and isolation of naturalBacterial infections are a global threat to human health1. Especially nosocomial infections with multidrug-resistant Gram-negative bacteria challenge public health systems and endanger successful treatment of patients in various modern medicine applications as e.g. organ transplant. In the last three decades classical approaches failed to find novel chemical scaffolds suitable for the development of antibiotics. Insect microbiomes produce potent antibiotics with low toxicity to eukaryotic cells to defend their hosts against entomopathogenic microorganisms2. In order to exploit insect microbiomes efficiently and to identify novel antibiotic producing bacteria, we implemented an high throughput Microfluidics/FACS (fluorescence-associated cell sorting) cultivation and screening pipeline. Here, we present the technical aspects of our pipeline including downstream processing. This includes genomic fingerprinting of identified producer strains, metabolomic analysis and isolation of natural products.zeige mehrzeige weniger

Volltext Dateien herunterladen

  • 2017-08-26_Poster_VAAM_Tuebingen.pdf
    eng

Metadaten exportieren

Weitere Dienste

Teilen auf Twitter Suche bei Google Scholar
Metadaten
Autoren/innen:Markus Oberpaul, Mai Ngyuen, Jochen Fracowiak, Florian Zubeil, Rüdiger Plarre, Peter Hammann, Andreas Vilcinskas, Jens Gläser
Dokumenttyp:Posterpräsentation
Veröffentlichungsform:Präsentation
Sprache:Englisch
Jahr der Erstveröffentlichung:2017
Organisationseinheit der BAM:4 Material und Umwelt
4 Material und Umwelt / 4.1 Biologische Materialschädigung und Referenzorganismen
DDC-Klassifikation:Technik, Medizin, angewandte Wissenschaften / Ingenieurwissenschaften / Sanitär- und Kommunaltechnik; Umwelttechnik
Freie Schlagwörter:Antibiotics; Microfluids; Termites
Themenfelder/Aktivitätsfelder der BAM:Umwelt
Veranstaltung:VAAM Workshop Biology of Bacteria Producing Natural Products
Veranstaltungsort:Tübingen, Germany
Beginndatum der Veranstaltung:27.09.2017
Enddatum der Veranstaltung:29.09.2017
Verfügbarkeit des Volltexts:Volltext-PDF im Netzwerk der BAM verfügbar ("Closed Access")
Datum der Freischaltung:04.09.2017
Referierte Publikation:Nein