- Treffer 1 von 1
Zitieren Sie bitte immer diesen URN: urn:nbn:de:kobv:b43-570205
Identification of Potential Antimicrobial Targets of Pseudomonas aeruginosa Biofilms through a Novel Screening Approach
- Pseudomonas aeruginosa is an opportunistic pathogen of considerable medical importance, owing to its pronounced antibiotic tolerance and association with cystic fibrosis and other life-threatening diseases. The aim of this study was to highlight the genes responsible for P. aeruginosa biofilm tolerance to antibiotics and thereby identify potential new targets for the development of drugs against biofilm-related infections. By developing a novel screening approach and utilizing a public P. aeruginosa transposon insertion library, several biofilm-relevant genes were identified. The Pf phage gene (PA0720) and flagellin gene (fliC) conferred biofilm-specific tolerance to gentamicin. Compared with the reference biofilms, the biofilms formed by PA0720 and fliC mutants were completely eliminated with a 4-fold-lower gentamicin concentration. Furthermore, the mreC, pprB, coxC, and PA3785 genes were demonstrated to play major roles in enhancing biofilm tolerance to gentamicin. The analysis ofPseudomonas aeruginosa is an opportunistic pathogen of considerable medical importance, owing to its pronounced antibiotic tolerance and association with cystic fibrosis and other life-threatening diseases. The aim of this study was to highlight the genes responsible for P. aeruginosa biofilm tolerance to antibiotics and thereby identify potential new targets for the development of drugs against biofilm-related infections. By developing a novel screening approach and utilizing a public P. aeruginosa transposon insertion library, several biofilm-relevant genes were identified. The Pf phage gene (PA0720) and flagellin gene (fliC) conferred biofilm-specific tolerance to gentamicin. Compared with the reference biofilms, the biofilms formed by PA0720 and fliC mutants were completely eliminated with a 4-fold-lower gentamicin concentration. Furthermore, the mreC, pprB, coxC, and PA3785 genes were demonstrated to play major roles in enhancing biofilm tolerance to gentamicin. The analysis of biofilm-relevant genes performed in this study provides important novel insights into the understanding of P. aeruginosa antibiotic tolerance, which will facilitate the detection of antibiotic resistance and the development of antibiofilm strategies against P. aeruginosa.…
Autor*innen: | Jules D. P. Valentin, Stefanie Altenried, Adithi R. Varadarajan, Christian H. Ahrens, Frank SchreiberORCiD, Jeremy S. Webb, Henny C. van der Mei, Qun Ren |
---|---|
Dokumenttyp: | Zeitschriftenartikel |
Veröffentlichungsform: | Verlagsliteratur |
Sprache: | Englisch |
Titel des übergeordneten Werkes (Englisch): | Microbiology Spectrum |
Jahr der Erstveröffentlichung: | 2023 |
Organisationseinheit der BAM: | 4 Material und Umwelt |
4 Material und Umwelt / 4.1 Biologische Materialschädigung und Referenzorganismen | |
Veröffentlichende Institution: | Bundesanstalt für Materialforschung und -prüfung (BAM) |
Verlag: | ASM Journals |
Aufsatznummer: | e0309922 |
Erste Seite: | 1 |
Letzte Seite: | 5 |
DDC-Klassifikation: | Naturwissenschaften und Mathematik / Chemie / Analytische Chemie |
Freie Schlagwörter: | Antimicrobial resistance; Bacteria; Biofilms; Pseudomonas aeruginosa |
Themenfelder/Aktivitätsfelder der BAM: | Umwelt |
DOI: | 10.1128/spectrum.03099-22 |
URN: | urn:nbn:de:kobv:b43-570205 |
Verfügbarkeit des Dokuments: | Datei für die Öffentlichkeit verfügbar ("Open Access") |
Lizenz (Deutsch): | Creative Commons - CC BY - Namensnennung 4.0 International |
Datum der Freischaltung: | 22.02.2023 |
Referierte Publikation: | Ja |
Datum der Eintragung als referierte Publikation: | 22.02.2023 |
Schriftenreihen ohne Nummerierung: | Wissenschaftliche Artikel der BAM |