Corundum as a novel affinity platform for the isolation of human IgG from plasma
- Nonporous corundum powder was functionalized covalently with protein binders to isolate and enrich specific proteins from complex matrices.The common crosslinker glutaraldehyde was compared with a hyperbranched polyglycerol (PG) of around 10 kDa. The latter was oxidized with periodate to generate aldehyde groups that can covalently react with the amines of the surface and the amino groups from the protein via a reductive amination process. The amount of bound protein was quantified via aromatic amino acid analysis (AAAA). As a proof of concept, IgG was extracted with protein A from crude human plasma. The advantages of corundum include the very low price, extremely high physical and chemical stability, pressure resistance, favorable binding kinetics, convenient handling, and flexible application.
Autor*innen: | Jule L. Völzke |
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Weitere Person: | Parya Hodjat Shamami, Kornelia Gawlitza, Ines Feldmann, Annett Zimathies, Klas MeyerORCiD, Michael G. WellerORCiD |
Dokumenttyp: | Posterpräsentation |
Veröffentlichungsform: | Präsentation |
Sprache: | Englisch |
Jahr der Erstveröffentlichung: | 2022 |
Organisationseinheit der BAM: | 1 Analytische Chemie; Referenzmaterialien |
1 Analytische Chemie; Referenzmaterialien / 1.4 Prozessanalytik | |
1 Analytische Chemie; Referenzmaterialien / 1.5 Proteinanalytik | |
1 Analytische Chemie; Referenzmaterialien / 1.9 Chemische und optische Sensorik | |
4 Material und Umwelt | |
4 Material und Umwelt / 4.2 Material-Mikrobiom Wechselwirkungen | |
6 Materialchemie | |
6 Materialchemie / 6.3 Strukturanalytik | |
DDC-Klassifikation: | Technik, Medizin, angewandte Wissenschaften / Ingenieurwissenschaften / Ingenieurwissenschaften und zugeordnete Tätigkeiten |
Freie Schlagwörter: | Affinity chromatography; Affinity extraction; Affinity support; Aluminum oxide; Amino acid analysis; Antibodies; Antibody purification; Aromatic amino acid analysis AAAA; BSA; Bovine serum albumin; Downstream processing; ESEM; Glutaraldehyde; Human plasma; Phosphonic acids; Polyglycerol; Protein a; Protein hydrolysis; Protein immobilization; Protein quantification; Reductive amination; SDS-PAGE; Sapphire; TEM; Tyrosine |
Themenfelder/Aktivitätsfelder der BAM: | Material |
Material / Materialdesign | |
Veranstaltung: | Adlershofer Forschungsforum 2022 |
Veranstaltungsort: | Berlin, Germany |
Beginndatum der Veranstaltung: | 11.11.2022 |
Verfügbarkeit des Dokuments: | Datei im Netzwerk der BAM verfügbar ("Closed Access") |
Datum der Freischaltung: | 02.11.2022 |
Referierte Publikation: | Nein |