Consequences of benzalkonium chloride tolerance in Escherichia coli: Effects on selection and evolution in the presence of ciprofloxacin
- We investigated the selection dynamics between a benzalkonium chloride (BAC)-tolerant Escherichia coli strain (S4) and a sensitive wild type under four conditions: in the absence of antibiotics and in the presence of three different sub-inhibitory concentrations of the antibiotic ciprofloxacin in liquid cultures. The wild type was selected over the BAC-tolerant strain in the absence of antibiotics, while the opposite was observed at all ciprofloxacin concentrations investigated.Furthermore, we assessed the evolvability of resistance of the two strains to inhibitory concentrations of ciprofloxacin by performing a serial dilution evolution experiment with gradually increasing ciprofloxacin concentrations. The wild type had a higher probability to develop resistance to ciprofloxacin than the tolerant strain. By the end of the evolution experiment both strains evolved to grow at the highest ciprofloxacin concentration investigated, which was 2048 ×MIC of the wild type.
Autor*innen: | Orestis Kanaris |
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Koautor*innen: | Niclas Nordholt, Frank Schreiber |
Dokumenttyp: | Posterpräsentation |
Veröffentlichungsform: | Präsentation |
Sprache: | Englisch |
Jahr der Erstveröffentlichung: | 2022 |
Organisationseinheit der BAM: | 4 Material und Umwelt |
4 Material und Umwelt / 4.1 Biologische Materialschädigung und Referenzorganismen | |
DDC-Klassifikation: | Technik, Medizin, angewandte Wissenschaften / Ingenieurwissenschaften / Sanitär- und Kommunaltechnik; Umwelttechnik |
Freie Schlagwörter: | Antimicrobial resistance; Experimental evolution; Selection; Tolerance |
Themenfelder/Aktivitätsfelder der BAM: | Umwelt |
Veranstaltung: | 6th international symposium on the environmental dimention of antibiotic resistance-EDAR 6 |
Veranstaltungsort: | Gothenburg, Sweden |
Beginndatum der Veranstaltung: | 22.09.2022 |
Enddatum der Veranstaltung: | 27.09.2022 |
Verfügbarkeit des Dokuments: | Datei im Netzwerk der BAM verfügbar ("Closed Access") |
Datum der Freischaltung: | 10.01.2023 |
Referierte Publikation: | Nein |