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Influence of femtosecond laser produced nanostructures on biofilm growth on steel

  • Large area periodic surface structures were generated on steel surfaces using 30-fs laser pulses at 790 nm wavelength. Two types of steel exhibiting a different corrosion resistance were used, i.e. a plain structural steel (corrodible) and a stainless steel (resistant to corrosion). Homogeneous fields of laser-induced periodic surface structures (LIPSS) were realized utilizing laser fluences close to the ablation threshold while scanning the sample under the focused laser beam in a multi-pulse regime. The nanostructures were characterized with optical and scanning electron microscopy. For each type of steel, more than ten dentical samples were laser-processed. These samples were subjected to microbial adhesion tests, investigating bacterial adhesion behavior on the laser structures in comparison to polished reference surfaces. Short term experiments (<24h) were carried out to determine initial biofilm development. E. coli as a typical bacterium representing pathogenic bacteria andLarge area periodic surface structures were generated on steel surfaces using 30-fs laser pulses at 790 nm wavelength. Two types of steel exhibiting a different corrosion resistance were used, i.e. a plain structural steel (corrodible) and a stainless steel (resistant to corrosion). Homogeneous fields of laser-induced periodic surface structures (LIPSS) were realized utilizing laser fluences close to the ablation threshold while scanning the sample under the focused laser beam in a multi-pulse regime. The nanostructures were characterized with optical and scanning electron microscopy. For each type of steel, more than ten dentical samples were laser-processed. These samples were subjected to microbial adhesion tests, investigating bacterial adhesion behavior on the laser structures in comparison to polished reference surfaces. Short term experiments (<24h) were carried out to determine initial biofilm development. E. coli as a typical bacterium representing pathogenic bacteria and Shewanella putrefaciens as metal corrosive bacterium were used for biofilm development analyses. Bacterial cell adhesion was determined microscopically after DAPI cell staining (DNA staining). Comparison of the coverage areas between nanostructured and polished surfaces revealed differences in cell adhesion behavior and biofilm structure.zeige mehrzeige weniger

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Metadaten
Autoren/innen:Nadja Epperlein
Koautoren/innen:Friederike Menzel, Karin Schwibbert, Robert Koter, Jörn Bonse, Janin Sameith, Jörg Krüger, Jörg Toepel
Dokumenttyp:Posterpräsentation
Veröffentlichungsform:Präsentation
Sprache:Englisch
Jahr der Erstveröffentlichung:2016
Organisationseinheit der BAM:4 Material und Umwelt
4 Material und Umwelt / 4.1 Biologische Materialschädigung und Referenzorganismen
6 Materialschutz und Oberflächentechnik
6 Materialschutz und Oberflächentechnik / 6.4 Technologien mit Nanowerkstoffen
DDC-Klassifikation:Technik, Medizin, angewandte Wissenschaften / Ingenieurwissenschaften / Ingenieurwissenschaften und zugeordnete Tätigkeiten
Freie Schlagwörter:Biofilms; Femtosecond laser ablation; Laser-induced periodic surface structures (LIPSS); Steel
Themenfelder/Aktivitätsfelder der BAM:Material
Veranstaltung:E-MRS 2016 Spring Meeting, Symposium C: "Laser-material interactions for tailoring future applications"
Veranstaltungsort:Lille, France
Beginndatum der Veranstaltung:02.05.2016
Enddatum der Veranstaltung:06.05.2016
Verfügbarkeit des Dokuments:Datei im Netzwerk der BAM verfügbar ("Closed Access")
Datum der Freischaltung:17.05.2016
Referierte Publikation:Nein