Zitieren Sie bitte immer diesen URN: urn:nbn:de:kobv:b43-404244
Measurements and simulations of microscopic damage to DNA in water by 30 keV electrons: A general approach applicable to other radiation sources and biological targets
- The determination of the microscopic dose-damage relationship for DNA in an aqueous environment is of a fundamental interest for dosimetry and applications in radiation therapy and protection. We combine geant4 particle-scattering simulations in water with calculations concerning the movement of biomolecules to obtain the energy deposit in the biologically relevant nanoscopic volume. We juxtaposition these results to the experimentally determined damage to obtain the dose-damage relationship at a molecular level. This approach is tested for an experimentally challenging system concerning the direct irradiation of plasmid DNA (pUC19) in water with electrons as primary particles. Here a microscopic target model for the plasmid DNA based on the relation of lineal energy and radiation quality is used to calculate the effective target volume. It was found that on average fewer than two ionizations within a 7.5-nm radius around the sugar-phosphate backbone are sufficient to cause a singleThe determination of the microscopic dose-damage relationship for DNA in an aqueous environment is of a fundamental interest for dosimetry and applications in radiation therapy and protection. We combine geant4 particle-scattering simulations in water with calculations concerning the movement of biomolecules to obtain the energy deposit in the biologically relevant nanoscopic volume. We juxtaposition these results to the experimentally determined damage to obtain the dose-damage relationship at a molecular level. This approach is tested for an experimentally challenging system concerning the direct irradiation of plasmid DNA (pUC19) in water with electrons as primary particles. Here a microscopic target model for the plasmid DNA based on the relation of lineal energy and radiation quality is used to calculate the effective target volume. It was found that on average fewer than two ionizations within a 7.5-nm radius around the sugar-phosphate backbone are sufficient to cause a single strand break, with a corresponding median lethal energy deposit being E1/2=6±4 eV. The presented method is applicable for ionizing radiation (e.g., γ rays, x rays, and electrons) and a variety of targets, such as DNA, proteins, or cells.…
Autor*innen: | Marc Benjamin HahnORCiD, Susann Meyer, Hans-Jörg KunteORCiD, Tihomir Solomun, Heinz SturmORCiD |
---|---|
Dokumenttyp: | Zeitschriftenartikel |
Veröffentlichungsform: | Verlagsliteratur |
Sprache: | Englisch |
Titel des übergeordneten Werkes (Englisch): | Physical Review E |
Jahr der Erstveröffentlichung: | 2017 |
Veröffentlichende Institution: | Bundesanstalt für Materialforschung und -prüfung (BAM) |
Herausgeber (Institution): | American Physical Society |
Verlag: | American Physical Society |
Verlagsort: | USA |
Jahrgang/Band: | 95 |
Ausgabe/Heft: | 5 |
Erste Seite: | 052419-1 |
Letzte Seite: | 052419-8 |
DDC-Klassifikation: | Naturwissenschaften und Mathematik / Chemie / Analytische Chemie |
Technik, Medizin, angewandte Wissenschaften / Ingenieurwissenschaften / Sanitär- und Kommunaltechnik; Umwelttechnik | |
Freie Schlagwörter: | DNA; DNA radiation damage; Dosimetry; Double-strand break (DSB); Geant4; Lethal dose; Low energy electrons; Microdosimetry; Monte Carlo simulation; Plasmid DNA in water; Radiation damage; Radiation damage to biomolecules; Single-strand break (SSB); Solutions (pH, salinity, cosolutes) |
DOI: | 10.1103/PhysRevE.95.052419 |
URN: | urn:nbn:de:kobv:b43-404244 |
ISSN: | 2470-0045 |
ISSN: | 2470-0053 |
Zugehöriger Identifikator: | https://doi.org/10.26272/opus4-55751 |
Verfügbarkeit des Dokuments: | Datei für die Öffentlichkeit verfügbar ("Open Access") |
Lizenz (Deutsch): | Creative Commons - Namensnennung |
Datum der Freischaltung: | 31.05.2017 |
Referierte Publikation: | Ja |
Datum der Eintragung als referierte Publikation: | 22.06.2017 |
Schriftenreihen ohne Nummerierung: | Wissenschaftliche Artikel der BAM |