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A similar secretome disturbance as a hallmark of non-pathogenic Botrytis cinerea ATMT-mutants?
- The gray mold fungus Botrytis cinerea is a necrotrophic pathogen able to infect hundreds of host plants, including high-value crops such as grapevine, strawberry and tomato. In order to decipher its infectious strategy, a library of 2,144 mutants was generated by random insertional mutagenesis using Agrobacterium tumefaciensmediated transformation (ATMT). Twelve mutants exhibiting total loss of virulence toward different host plants were chosen for detailed analyses. Their molecular characterization revealed a single T-DNA insertion in different loci. Using a proteomics approach, the secretome of four of these strains was compared to that of the parental strain and a common profile of reduced lytic enzymes was recorded. Significant variations in this profile, notably deficiencies in the secretion of proteases and hemicellulases, were observed and validated by biochemical tests. They were also a hallmark of the remaining eight non-pathogenic strains, suggesting the importance of theseThe gray mold fungus Botrytis cinerea is a necrotrophic pathogen able to infect hundreds of host plants, including high-value crops such as grapevine, strawberry and tomato. In order to decipher its infectious strategy, a library of 2,144 mutants was generated by random insertional mutagenesis using Agrobacterium tumefaciensmediated transformation (ATMT). Twelve mutants exhibiting total loss of virulence toward different host plants were chosen for detailed analyses. Their molecular characterization revealed a single T-DNA insertion in different loci. Using a proteomics approach, the secretome of four of these strains was compared to that of the parental strain and a common profile of reduced lytic enzymes was recorded. Significant variations in this profile, notably deficiencies in the secretion of proteases and hemicellulases, were observed and validated by biochemical tests. They were also a hallmark of the remaining eight non-pathogenic strains, suggesting the importance of these secreted Proteins in the infection process. In the twelve non-pathogenic mutants, the Differentiation of infection cushions was also impaired, suggesting a link between the Penetration structures and the secretion of proteins involved in the virulence of the pathogen.…
Autor*innen: | A. de Vallée, P. Bally, C. Bruel, L. Chandat, M. Choquer, C. Dieryckx, J.W. Dupuy, S. Kaiser, M.-P. Latorse, E. Loisel, G. Mey, G. Morgant, C. Rascle, Julia SchumacherORCiD, A. Simon, E. Souibgui, M. Viaud, F. Villalba, N. Poussereau |
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Dokumenttyp: | Zeitschriftenartikel |
Veröffentlichungsform: | Verlagsliteratur |
Sprache: | Englisch |
Titel des übergeordneten Werkes (Englisch): | Frontiers in microbiology |
Jahr der Erstveröffentlichung: | 2019 |
Organisationseinheit der BAM: | 4 Material und Umwelt |
4 Material und Umwelt / 4.0 Abteilungsleitung und andere | |
Veröffentlichende Institution: | Bundesanstalt für Materialforschung und -prüfung (BAM) |
Verlag: | Frontiers Media |
Verlagsort: | Lausanne |
Jahrgang/Band: | 10 |
Erste Seite: | Article 2829 |
DDC-Klassifikation: | Technik, Medizin, angewandte Wissenschaften / Ingenieurwissenschaften / Sanitär- und Kommunaltechnik; Umwelttechnik |
Freie Schlagwörter: | Fungus; Plant pathogen; Virulence |
Themenfelder/Aktivitätsfelder der BAM: | Umwelt |
Umwelt / Umwelt-Material-Interaktionen | |
DOI: | 10.3389/fmicb.2019.02829 |
URN: | urn:nbn:de:kobv:b43-502710 |
ISSN: | 1664-302X |
Verfügbarkeit des Dokuments: | Datei für die Öffentlichkeit verfügbar ("Open Access") |
Lizenz (Deutsch): | Creative Commons - CC BY - Namensnennung 4.0 International |
Datum der Freischaltung: | 23.01.2020 |
Referierte Publikation: | Ja |
Datum der Eintragung als referierte Publikation: | 23.01.2020 |
Schriftenreihen ohne Nummerierung: | Wissenschaftliche Artikel der BAM |