Troubleshooting for the clinical measurement of iron biomarkers using LC-MS/MS: Suppression of sample losses in autosampler vials
- We developed a rapid and robust HPLC-MS/MS (QqQ) method for the quantification of hepcidin-25, a promising new biomarker in iron metabolism, in human samples. The novelty of the method is the use of special HPLC vials to avoid adsorptive losses due to the basic character of the peptide that causes interaction with the silanol groups of the vial’s glass surface. Up to 90% decrease in the MS/MS signal was observed, when commercial HPLC vials were used, while vials treated with 3-(2-aminoethylamino)propylmethyldimethoxysilane or 1H,1H,2H,2H-perfluorooctyltriethoxysilane, leading to no losses in the range of physiological hepcidin-25 mean serum levels (10-20 µg/L).
Autor*innen: | Ioana M. AbbasORCiD |
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Koautor*innen: | Holger Hoffmann, Michael G. Weller |
Dokumenttyp: | Posterpräsentation |
Veröffentlichungsform: | Präsentation |
Sprache: | Englisch |
Jahr der Erstveröffentlichung: | 2017 |
DDC-Klassifikation: | Naturwissenschaften und Mathematik / Chemie / Analytische Chemie |
Freie Schlagwörter: | Adsorption; Peptide losses; Silanization |
Veranstaltung: | MSACL 2017 EU |
Veranstaltungsort: | Salzburg, Austria |
Beginndatum der Veranstaltung: | 10.09.2017 |
Verfügbarkeit des Dokuments: | Datei im Netzwerk der BAM verfügbar ("Closed Access") |
Datum der Freischaltung: | 04.04.2018 |
Referierte Publikation: | Nein |