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Microfluidic Paper-Based Analytical Devices with Surface Enhanced Raman Scattering Detection

  • Microfluidic paper-based analytical devices (μPADs) in combination with surface enhanced Raman scattering (SERS) provide a way for analyses of complex mixtures. The μPADs can be used for the chromatographic separation of different compounds of mixtures in combination with the separate detection of the analytes in different zones on the paper by SERS. SERS allows to observe analytes directly without labelling in low concentrations in aqueous solutions and to identify them by their spectral fingerprint. SERS substrates on the μPADs were created by drying standard silver nanoparticle (AgNP) solution on the paper. The microfluidic structure of the μPADs was prepared by wax printing. As a model system, an aqueous solution of the non-fluorescent analyte adenine and two fluorescent dyes tris(2,2’-bipyridyl)dichlororuthenium(II) and sulforhodamine B was tested. The dependency of the SERS signal intensity on the analyte concentration can be fitted using a Langmuir isotherm curve progression.Microfluidic paper-based analytical devices (μPADs) in combination with surface enhanced Raman scattering (SERS) provide a way for analyses of complex mixtures. The μPADs can be used for the chromatographic separation of different compounds of mixtures in combination with the separate detection of the analytes in different zones on the paper by SERS. SERS allows to observe analytes directly without labelling in low concentrations in aqueous solutions and to identify them by their spectral fingerprint. SERS substrates on the μPADs were created by drying standard silver nanoparticle (AgNP) solution on the paper. The microfluidic structure of the μPADs was prepared by wax printing. As a model system, an aqueous solution of the non-fluorescent analyte adenine and two fluorescent dyes tris(2,2’-bipyridyl)dichlororuthenium(II) and sulforhodamine B was tested. The dependency of the SERS signal intensity on the analyte concentration can be fitted using a Langmuir isotherm curve progression. With this approach, a semi-quantitative analysis of the components is possible. The reproducibility and stability of the measurement procedure was tested with several measurements over time, different NP batches, and with different analytes in different concentrations and resulted in an average relative standard deviation of 16 %. The SERS spectra of the mixture of the model system are dominated by one compound depending on the concentration ratio. For the detection and identification of all components of the mixture, the compounds were therefore separated on the μPADs and measured at different positions. The position of adenine on the μPADs is dependent on the AgNP coverage of the paper. Due to this effect, it possible to detect adenine on a defined point on the μPADs and to get an information on the concentration in a mixture of three components.zeige mehrzeige weniger

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Metadaten
Autor*innen:Axel Bolz
Koautor*innen:Knut Rurack, Merwe Buurman
Dokumenttyp:Posterpräsentation
Veröffentlichungsform:Präsentation
Sprache:Englisch
Jahr der Erstveröffentlichung:2017
DDC-Klassifikation:Naturwissenschaften und Mathematik / Chemie / Analytische Chemie
Freie Schlagwörter:SERS; µPAD
Veranstaltung:30. Tag der Chemie Humboldt-Universität zu Berlin
Veranstaltungsort:Berlin, Germany
Beginndatum der Veranstaltung:05.07.2017
Enddatum der Veranstaltung:05.07.2017
Verfügbarkeit des Dokuments:Datei im Netzwerk der BAM verfügbar ("Closed Access")
Datum der Freischaltung:04.10.2017
Referierte Publikation:Nein
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