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Immunomagnetic ochratoxin A assay with smartphone-based amperometric detection

  • Toxic secondary metabolites produced by fungi (aka mycotoxins) are often identified as contaminants in food and pose a severe health risk. Ochratoxin A (OTA) is one of the most abundant mycotoxins found in grains such as wheat, rye or oats. Thus, the fast and cost-effective detection of OTA is of great importance, to ensure that the toxin concentration is below the legal limits. Currently, mycotoxin detection in mill operations is typically performed by means of the antibody-based ELISA (Enzyme-linked Immunosorbent Assay) method, which requires extensive laboratory equipment and specialized personnel. The aim of this project is to establish a more user-friendly detection method. Thus, we developed a magnetic bead-based assay enabling the quantification of OTA with a miniaturized potentiostat connected to a smartphone.

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Autoren/innen:Soraya Höfs
Koautoren/innen:Peter Carl, Rudolf Schneider
Jahr der Erstveröffentlichung:2020
Organisationseinheit der BAM:1 Analytische Chemie; Referenzmaterialien
1 Analytische Chemie; Referenzmaterialien / 1.8 Umweltanalytik
DDC-Klassifikation:Naturwissenschaften und Mathematik / Chemie / Analytische Chemie
Freie Schlagwörter:amperometry; cyclic voltammetry; magnetic beads; ochratoxin A; screen-printed electrodes; smartphone
Themenfelder/Aktivitätsfelder der BAM:Analytical Sciences
Analytical Sciences / Sensorik
Veranstaltung:SALSA Make and Measure 2020
Veranstaltungsort:Online meeting
Beginndatum der Veranstaltung:15.10.2020
Enddatum der Veranstaltung:17.10.2020
Verfügbarkeit des Dokuments:Datei im Netzwerk der BAM verfügbar ("Closed Access")
Datum der Freischaltung:21.10.2020
Referierte Publikation:Nein