Characterization of metal-tagged antibodies used in ICP-MS-based immunoassays
- A detailed characterization of metal-tagged antibodies is the prerequisite for the implementation of quantitative concepts in inductively coupled plasma–mass spectrometry (ICP-MS)-based bioanalysis or future medical diagnosis. In this paper, the common modification with bifunctional ligands containing maleimide residues as a reactive group was investigated in detail via size exclusion chromatography (SEC)-ICP-MS and liquid chromatography–time-of-flight (LC-TOF)-MS to determine the preservation of the antibody structure after tagging. Mouse monoclonal IgG modified with metal-coded tags (MeCATs) was used as a model system. Several antibody fragments were identified carrying different numbers of metal tags. In a second step, a functionality test was performed with isolated fragments where the antigen specificity was tested in a dot blot immunoassay.
Autor*innen: | Larissa Müller, T. Mairinger, G. Hermann, G. Koellensperger, S. Hann |
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Dokumenttyp: | Zeitschriftenartikel |
Veröffentlichungsform: | Verlagsliteratur |
Sprache: | Englisch |
Titel des übergeordneten Werkes (Englisch): | Analytical and bioanalytical chemistry |
Jahr der Erstveröffentlichung: | 2014 |
Verlag: | Springer |
Verlagsort: | Berlin |
Jahrgang/Band: | 406 |
Ausgabe/Heft: | 1 |
Erste Seite: | 163 |
Letzte Seite: | 169 |
Freie Schlagwörter: | Antibodies; Antibody tagging; Bioanalytical methods; ICP-MS; Immunoassay; Maleimide DOTA; Mass spectrometry; Tags |
DOI: | 10.1007/s00216-013-7416-x |
ISSN: | 1618-2642 |
ISSN: | 1618-2650 |
Verfügbarkeit des Dokuments: | Datei im Netzwerk der BAM verfügbar ("Closed Access") |
Datum der Freischaltung: | 20.02.2016 |
Referierte Publikation: | Ja |
Datum der Eintragung als referierte Publikation: | 23.01.2014 |