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In situ monitoring of the influence of water on DNA radiation damage by near-ambient pressure X-ray photoelectron spectroscopy
- Ionizing radiation damage to DNA plays a fundamental role in cancer therapy. X-ray photoelectron-spectroscopy (XPS) allows simultaneous irradiation and damage monitoring. Although water radiolysis is essential for radiation damage, all previous XPS studies were performed in vacuum. Here we present near-ambient-pressure XPS xperiments to directly measure DNA damage under water atmosphere. They permit in-situ monitoring of the effects of radicals on fully hydrated double-stranded DNA. The results allow us to distinguish direct damage, by photons and secondary low-energy electrons (LEE), from damage by hydroxyl radicals or hydration induced modifications of damage pathways. The exposure of dry DNA to x-rays leads to strand-breaks at the sugar-phosphate backbone, while deoxyribose and nucleobases are less affected. In contrast, a strong increase of DNA damage is observed in water, where OH-radicals are produced. In consequence, base damage and base release become predominant, even thoughIonizing radiation damage to DNA plays a fundamental role in cancer therapy. X-ray photoelectron-spectroscopy (XPS) allows simultaneous irradiation and damage monitoring. Although water radiolysis is essential for radiation damage, all previous XPS studies were performed in vacuum. Here we present near-ambient-pressure XPS xperiments to directly measure DNA damage under water atmosphere. They permit in-situ monitoring of the effects of radicals on fully hydrated double-stranded DNA. The results allow us to distinguish direct damage, by photons and secondary low-energy electrons (LEE), from damage by hydroxyl radicals or hydration induced modifications of damage pathways. The exposure of dry DNA to x-rays leads to strand-breaks at the sugar-phosphate backbone, while deoxyribose and nucleobases are less affected. In contrast, a strong increase of DNA damage is observed in water, where OH-radicals are produced. In consequence, base damage and base release become predominant, even though the number of strand-breaks increases further.…
Autor*innen: | Marc Benjamin HahnORCiD, P. M. Dietrich, Jörg RadnikORCiD |
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Dokumenttyp: | Zeitschriftenartikel |
Veröffentlichungsform: | Verlagsliteratur |
Sprache: | Englisch |
Titel des übergeordneten Werkes (Englisch): | Communications Chemistry |
Jahr der Erstveröffentlichung: | 2021 |
Organisationseinheit der BAM: | 6 Materialchemie |
6 Materialchemie / 6.1 Oberflächen- und Dünnschichtanalyse | |
6 Materialchemie / 6.6 Physik und chemische Analytik der Polymere | |
Veröffentlichende Institution: | Bundesanstalt für Materialforschung und -prüfung (BAM) |
Verlag: | Springer Nature |
Verlagsort: | London |
Jahrgang/Band: | 4 |
Ausgabe/Heft: | 1 |
Erste Seite: | 50 |
DDC-Klassifikation: | Naturwissenschaften und Mathematik / Chemie / Analytische Chemie |
Technik, Medizin, angewandte Wissenschaften / Ingenieurwissenschaften / Ingenieurwissenschaften und zugeordnete Tätigkeiten | |
Freie Schlagwörter: | Base damage; Base loss; Cancer therapy; DNA; DNA radiation damage; Direct damage; Dissociative electron attachment (DEA); Dissociative electron transfer (DET); Dosimetry; Double-strand break (DSB); Dry DNA; Geant4; Geant4-DNA; Hydrated DNA; Hydrated electron; Hydration shell; Hydroxyl radical; Indirect damage; Ionization; LEE; Low energy electrons; Microdosimetry; NAP-XPS; Near ambient pressure xray photo electron spectroscopy; Net-ionization reaction; OH radical; PES; Prehydrated electron; Quasi-direct damage; ROS; Radiation damage; Radiation therapy; Radical; Reactive oxygen species; Single-strand break (SSB); TOPAS; TOPAS-nbio; XPS; Xray; Xray photo electron spectrocopy |
Themenfelder/Aktivitätsfelder der BAM: | Chemie und Prozesstechnik |
Material | |
DOI: | 10.1038/s42004-021-00487-1 |
URN: | urn:nbn:de:kobv:b43-524060 |
ISSN: | 2399-3669 |
Zugehöriger Identifikator: | https://opus4.kobv.de/opus4-bam/frontdoor/index/index/docId/52461 |
Verfügbarkeit des Dokuments: | Datei für die Öffentlichkeit verfügbar ("Open Access") |
Lizenz (Deutsch): | Creative Commons - CC BY - Namensnennung 4.0 International |
Datum der Freischaltung: | 12.04.2021 |
Referierte Publikation: | Ja |
Datum der Eintragung als referierte Publikation: | 29.04.2021 |