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Development of highly sensitive and selective antibodies for the detection of the explosive pentaerythritol tetranitrate (PETN) by bioisosteric replacement

  • An improved antibody against the explosive pentaerythritol tetranitrate (PETN) was developed. The immunogen was designed by the concept of bioisosteric replacement, which led to an excellent polyclonal antibody with extreme selectivity and immunoassays of very good sensitivity. Compounds such as nitroglycerine, 2,4,6-trinitrotoluene, 1,3,5-trinitrobenzene, hexogen (RDX), 2,4,6-trinitroaniline, 1,3-dinitrobenzene, octogen (HMX), triacetone triperoxide (TATP), ammonium nitrate, 2,4,6-trinitrophenol and nitrobenzene were tested for potential cross-reactivity. The detection limit of a competitive enzyme-linked immunosorbent assay (ELISA) was determined to be around 0.5 µg/L. The dynamic range of the assay was found to be between 1 µg/L and 1000 µg/L, covering a concentration range of three decades. This work shows the successful application of the bioisosteric concept in immunochemistry by exchange of a nitroester to a carbonate diester. The antiserum might be used for the development ofAn improved antibody against the explosive pentaerythritol tetranitrate (PETN) was developed. The immunogen was designed by the concept of bioisosteric replacement, which led to an excellent polyclonal antibody with extreme selectivity and immunoassays of very good sensitivity. Compounds such as nitroglycerine, 2,4,6-trinitrotoluene, 1,3,5-trinitrobenzene, hexogen (RDX), 2,4,6-trinitroaniline, 1,3-dinitrobenzene, octogen (HMX), triacetone triperoxide (TATP), ammonium nitrate, 2,4,6-trinitrophenol and nitrobenzene were tested for potential cross-reactivity. The detection limit of a competitive enzyme-linked immunosorbent assay (ELISA) was determined to be around 0.5 µg/L. The dynamic range of the assay was found to be between 1 µg/L and 1000 µg/L, covering a concentration range of three decades. This work shows the successful application of the bioisosteric concept in immunochemistry by exchange of a nitroester to a carbonate diester. The antiserum might be used for the development of quick tests, biosensors, microtitration plate immunoassays, microarrays and other analytical methods for the highly sensitive detection of PETN, an explosive frequently used by terrorists, exploiting the extreme difficulty of its detection.zeige mehrzeige weniger

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Metadaten
Autor*innen:Almut Hesse, Mustafa Biyikal, Knut RurackORCiD, Michael G. WellerORCiD
Dokumenttyp:Zeitschriftenartikel
Veröffentlichungsform:Verlagsliteratur
Sprache:Englisch
Titel des übergeordneten Werkes (Englisch):Journal of Molecular Recognition
Jahr der Erstveröffentlichung:2016
Verlag:John Wiley & Sons, Ltd.
Verlagsort:New York, USA
Jahrgang/Band:29
Erste Seite:88
Letzte Seite:94
DDC-Klassifikation:Naturwissenschaften und Mathematik / Chemie / Analytische Chemie
Freie Schlagwörter:ELISA; Semtex; antibody; bioisosteric replacement; explosives; hapten; immunoassay; security; terrorism
DOI:10.1002/jmr.2511
URL:http://onlinelibrary.wiley.com/doi/10.1002/jmr.2511/abstract
ISSN:1099-1352
Verfügbarkeit des Dokuments:Datei im Netzwerk der BAM verfügbar ("Closed Access")
Datum der Freischaltung:27.04.2016
Referierte Publikation:Ja
Datum der Eintragung als referierte Publikation:27.04.2016
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