Comparison of ICP-MS and photometric detection of an immunoassay for the determination of ochratoxin A in wine
- An immunoassay was developed for ochratoxin A (OTA) detection in wine and two completely different detection methods were compared: ICP-MS and photometry. For labelling, we applied secondary antibodies conjugated with gold nanoparticles for ICP-MS and with horseradish peroxidase for photometric detection, respectively. Detection limits of the assay were determined as 0.003 µg L-1 for both detection methods. OTA in wine was determined below the EU limit value of 2 µg kg-1. The measurement range was between 0.01 and 1 µg L-1 for ICP-MS and photometric detection. Assay precision detected by ICP-MS and photometry were similar showing that precision of the detection method has only a minor effect on total assay precision. By using BSA as a buffer additive to minimize nonspecific binding, we could also confirm that OTA strongly binds to BSA. In a comparison study of several additives, casein was found to be preferable.
Autor*innen: | Charlotte Giesen, Norbert JakubowskiORCiD, Ulrich PanneORCiD, Michael G. WellerORCiD |
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Dokumenttyp: | Zeitschriftenartikel |
Veröffentlichungsform: | Verlagsliteratur |
Sprache: | Englisch |
Titel des übergeordneten Werkes (Englisch): | Journal of analytical atomic spectrometry |
Jahr der Erstveröffentlichung: | 2010 |
Verlag: | Royal Society of Chemistry |
Verlagsort: | London |
Jahrgang/Band: | 25 |
Ausgabe/Heft: | 10 |
Erste Seite: | 1567 |
Letzte Seite: | 1572 |
DOI: | 10.1039/c0ja00009d |
ISSN: | 0267-9477 |
ISSN: | 1364-5544 |
Verfügbarkeit des Dokuments: | Physisches Exemplar in der Bibliothek der BAM vorhanden ("Hardcopy Access") |
Bibliotheksstandort: | Sonderstandort: Publica-Schrank |
Datum der Freischaltung: | 19.02.2016 |
Referierte Publikation: | Ja |
Datum der Eintragung als referierte Publikation: | 09.12.2010 |