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Reference Materials for Standardization of Fluorescence-based Measurements

  • Here, we summerize our efforts concerning new design concepts and examples for fluorescence standards that can provide traceability to radiometric units and present a first step towards a toolbox of fluorescence standards, currently consisting of: i) A first set of liquid fluorescence standards enables the determination of a broad variety of fluorescence parameters was developed and certified by BAM and is distributed by Sigma-Aldrich. ii) Ready-to-use, glass-based fluorescence standards for instrument performance validation (IPV) and determination instrument-to-instrument variations can also be used as wavelength standard for fluorescence instruments with low requirements on spectral resolution and allow monitoring of temporal changes of the wavelength-dependent spectral responsivity. iii) Novel calibration tools and validation concepts for microarray-based platforms used in molecular diagnostics and food safety control. iv) We currently develop reference materials, which can beHere, we summerize our efforts concerning new design concepts and examples for fluorescence standards that can provide traceability to radiometric units and present a first step towards a toolbox of fluorescence standards, currently consisting of: i) A first set of liquid fluorescence standards enables the determination of a broad variety of fluorescence parameters was developed and certified by BAM and is distributed by Sigma-Aldrich. ii) Ready-to-use, glass-based fluorescence standards for instrument performance validation (IPV) and determination instrument-to-instrument variations can also be used as wavelength standard for fluorescence instruments with low requirements on spectral resolution and allow monitoring of temporal changes of the wavelength-dependent spectral responsivity. iii) Novel calibration tools and validation concepts for microarray-based platforms used in molecular diagnostics and food safety control. iv) We currently develop reference materials, which can be used as reliable quantum yield standards for relative methods for the determination of QY and can be valuable in the evaluation of the performance and sources of uncertainty of absolute, standard-free methods using e.g. integrating spheres.zeige mehrzeige weniger

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Metadaten
Autor*innen:Katrin HoffmannORCiD
Koautor*innen:Angelika Hoffmann, Jutta Pauli, Monika Spieles, Claudia Brunner, Ute Resch-Genger
Dokumenttyp:Posterpräsentation
Veröffentlichungsform:Präsentation
Sprache:Englisch
Jahr der Erstveröffentlichung:2015
DDC-Klassifikation:Naturwissenschaften und Mathematik / Chemie / Analytische Chemie
Freie Schlagwörter:Fluorescence; Microarray; Quantum yield; Standards
Veranstaltung:14th Conference on Methods and Applications in Fluorescence
Veranstaltungsort:Würzburg, Germany
Beginndatum der Veranstaltung:13.09.2015
Enddatum der Veranstaltung:16.09.2015
Verfügbarkeit des Dokuments:Datei im Netzwerk der BAM verfügbar ("Closed Access")
Datum der Freischaltung:04.01.2017
Referierte Publikation:Nein
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