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Electrochemistry-mass spectrometry (EC-MS) as a versatile tool to simulate the biotransformation of citrinin – A comparative study

  • Mycotoxins can be found worldwide in foods and feed and cause a variety of mold-related health risks which makes it necessary to further examine their toxic effects and metabolic fate in human and other mammals. Beside standard in vitro and in vivo assays with liver cell preparations or rodents an increasing interest in new simulation methods are playing a growing role. Electrochemistry (EC) is one of these novel techniques and has been used successfully and efficiently in pharmacological and drug research for several years now. The primary objective of this study was to determine the capability of EC as a supportive and versatile instrument to elucidate metabolic pathways of mycotoxins. On the example of the food relevant mycotoxin Citrinin a coulometric flow through cell equipped with a carbon working electrode was used to oxidize Citrinin by applying potential between 0.7 and 2.5 V vs. Pd/H2. The electrochemically generated oxidation products were then analyzed byMycotoxins can be found worldwide in foods and feed and cause a variety of mold-related health risks which makes it necessary to further examine their toxic effects and metabolic fate in human and other mammals. Beside standard in vitro and in vivo assays with liver cell preparations or rodents an increasing interest in new simulation methods are playing a growing role. Electrochemistry (EC) is one of these novel techniques and has been used successfully and efficiently in pharmacological and drug research for several years now. The primary objective of this study was to determine the capability of EC as a supportive and versatile instrument to elucidate metabolic pathways of mycotoxins. On the example of the food relevant mycotoxin Citrinin a coulometric flow through cell equipped with a carbon working electrode was used to oxidize Citrinin by applying potential between 0.7 and 2.5 V vs. Pd/H2. The electrochemically generated oxidation products were then analyzed by mass-spectrometric detection coupled online to EC (EC-MS) and compared with data from a standard in vitro model with human and rat liver microsomes preparations. To receive a comprehensive assessment of oxidative techniques chemical oxidation by Fenton´s reaction was performed as well. The obtained LC-MS/MS data confirmed the production of Dihydrocitrinone by all of the three tested oxidation systems and demonstrates the potential of EC-MS for the successful prediction of the main phase I metabolic reactions of mycotoxins, since Dihydrocitrinone is the mainly formed metabolite by humans after intake of Citrinin. Beside the identified Dihydrocitrinone from electrochemical, enzymatic and chemical oxidation of Citrinin there is still a number of yet unknown compounds. As the next step structural characterization of the generated oxidation products by NMR and X-ray analysis will be enabled by their large-scale production using preparative EC cells.zeige mehrzeige weniger

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Metadaten
Autoren/innen:Julia Keller
Koautoren/innen:H. Haase, Matthias Koch
Dokumenttyp:Posterpräsentation
Veröffentlichungsform:Präsentation
Sprache:Englisch
Jahr der Erstveröffentlichung:2016
Organisationseinheit der BAM:1 Analytische Chemie; Referenzmaterialien
1 Analytische Chemie; Referenzmaterialien / 1.7 Organische Spuren- und Lebensmittelanalytik
DDC-Klassifikation:Naturwissenschaften und Mathematik / Chemie / Analytische Chemie
Freie Schlagwörter:Electrochemistry; Mycotoxin; Oxidation
Themenfelder/Aktivitätsfelder der BAM:Analytical Sciences
Veranstaltung:Rapid Methods
Veranstaltungsort:Amsterdam, The Netherlands
Beginndatum der Veranstaltung:07.11.2016
Enddatum der Veranstaltung:09.11.2016
Verfügbarkeit des Dokuments:Datei im Netzwerk der BAM verfügbar ("Closed Access")
Datum der Freischaltung:11.11.2016
Referierte Publikation:Nein