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Fluorescence-based multiplexed detection on single test strips with antibody-gated mesoporous materials

  • Rapid testing methods for the use directly at a point-of-need are expected to unfold their true potential especially when offering adequate capabilities for the simultaneous measurement of multiple analytes of interest. However, the implementation of multiplexing features while retaining simplicity, performance and portability is one of the prominent challenges in the field. Keeping in mind these challenges, we decided to combine the use of stimuli-responsive materials for small-molecule sensing relying on chemical signal amplification and their incorporation on paper strips for lateral-flow assays in a straightforward manner. Considering the modularity, high sensitivity and selectivity of antibody-gated indicator delivery systems, a multiplexed assay for three small-molecule explosives TATP, TNT and PETN was developed, allowing to detect the analytes simultaneously with a single test strip at lower ppb concentrations in liquid phase in <5 min, using a fluorescence reader or aRapid testing methods for the use directly at a point-of-need are expected to unfold their true potential especially when offering adequate capabilities for the simultaneous measurement of multiple analytes of interest. However, the implementation of multiplexing features while retaining simplicity, performance and portability is one of the prominent challenges in the field. Keeping in mind these challenges, we decided to combine the use of stimuli-responsive materials for small-molecule sensing relying on chemical signal amplification and their incorporation on paper strips for lateral-flow assays in a straightforward manner. Considering the modularity, high sensitivity and selectivity of antibody-gated indicator delivery systems, a multiplexed assay for three small-molecule explosives TATP, TNT and PETN was developed, allowing to detect the analytes simultaneously with a single test strip at lower ppb concentrations in liquid phase in <5 min, using a fluorescence reader or a smartphone for readout. Because of the versatility of the hybrid material and the modularity of the assay architecture, it is obvious that this generic approach should be easily transferable to food or environmental analysis, point-of-care diagnostics and other areas of application in which the rapid screening for multiple parameters from liquid samples without clean-up in a dedicated laboratory is in demand.zeige mehrzeige weniger

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Metadaten
Autor*innen:Estela Climent TerolORCiD
Koautor*innen:Mustafa Biyikal, Delia Gröninger, Michael G. Weller, Ramón Martínez-Máñez, Knut Rurack
Dokumenttyp:Vortrag
Veröffentlichungsform:Präsentation
Sprache:Englisch
Jahr der Erstveröffentlichung:2021
Organisationseinheit der BAM:1 Analytische Chemie; Referenzmaterialien
1 Analytische Chemie; Referenzmaterialien / 1.9 Chemische und optische Sensorik
DDC-Klassifikation:Naturwissenschaften und Mathematik / Chemie / Analytische Chemie
Freie Schlagwörter:Explosives; Hybrid materials; Multiplexing; Signal amplification; Test Strip analysis
Themenfelder/Aktivitätsfelder der BAM:Umwelt
Umwelt / Sensorik
Veranstaltung:2021 On-line Symposium on Bioluminiscence, Chemiluminiscence and luminescence spectrometry ISLS-ISBC
Veranstaltungsort:Online meeting
Beginndatum der Veranstaltung:24.06.2021
Enddatum der Veranstaltung:24.06.2021
Verfügbarkeit des Dokuments:Datei im Netzwerk der BAM verfügbar ("Closed Access")
Datum der Freischaltung:30.06.2021
Referierte Publikation:Nein
Eingeladener Vortrag:Nein
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