- Treffer 1 von 1
Zitieren Sie bitte immer diesen URN: urn:nbn:de:kobv:b43-438873
Cell-to-cell variation and specialization in sugar metabolism in clonal bacterial populations
- While we have good understanding of bacterial metabolism at the population level, we know little about the metabolic behavior of individual cells: do single cells in clonal populations sometimes specialize on different metabolic pathways? Such metabolic specialization could be driven by stochastic gene expression and could provide individual cells with growth benefits of specialization. We measured the degree of phenotypic specialization in two parallel metabolic pathways, the assimilation of glucose and arabinose. We grew Escherichia coli in chemostats, and used isotope-labeled sugars in combination with nanometer-scale secondary ion mass spectrometry and mathematical modeling to quantify sugar assimilation at the single-cell level. We found large variation in metabolic activities between single cells, both in absolute assimilation and in the degree to which individual cells specialize in the assimilation of different sugars. Analysis of transcriptional reporters indicated that thisWhile we have good understanding of bacterial metabolism at the population level, we know little about the metabolic behavior of individual cells: do single cells in clonal populations sometimes specialize on different metabolic pathways? Such metabolic specialization could be driven by stochastic gene expression and could provide individual cells with growth benefits of specialization. We measured the degree of phenotypic specialization in two parallel metabolic pathways, the assimilation of glucose and arabinose. We grew Escherichia coli in chemostats, and used isotope-labeled sugars in combination with nanometer-scale secondary ion mass spectrometry and mathematical modeling to quantify sugar assimilation at the single-cell level. We found large variation in metabolic activities between single cells, both in absolute assimilation and in the degree to which individual cells specialize in the assimilation of different sugars. Analysis of transcriptional reporters indicated that this variation was at least partially based on cell-to-cell variation in gene expression. Metabolic differences between cells in clonal populations could potentially reduce metabolic incompatibilities between different pathways, and increase the rate at which parallel reactions can be performed.…
Autor*innen: | Nela Nikolic, Alma Dal Co, Daniel J. Kiviet, Tobias Bergmiller, Sten Littmann, Marcel M. M. Kuypers, Martin Ackermann, Frank SchreiberORCiD |
---|---|
Dokumenttyp: | Zeitschriftenartikel |
Veröffentlichungsform: | Verlagsliteratur |
Sprache: | Englisch |
Titel des übergeordneten Werkes (Englisch): | PLoS Genetics |
Jahr der Erstveröffentlichung: | 2017 |
Veröffentlichende Institution: | Bundesanstalt für Materialforschung und -prüfung (BAM) |
Verlag: | Public Library of Science |
Verlagsort: | Cambridge, United Kingdom |
Jahrgang/Band: | 13 |
Ausgabe/Heft: | 12 |
Erste Seite: | e1007122, 1 |
Letzte Seite: | e1007122, 24 |
DDC-Klassifikation: | Technik, Medizin, angewandte Wissenschaften / Ingenieurwissenschaften / Sanitär- und Kommunaltechnik; Umwelttechnik |
Freie Schlagwörter: | Escherichia coli; Metabolism; Phenotypic diversity; Phenotypic heterogeneity |
DOI: | 10.1371/journal.pgen.1007122 |
URN: | urn:nbn:de:kobv:b43-438873 |
URL: | http://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1007122 |
ISSN: | 1553-7404 |
Verfügbarkeit des Dokuments: | Datei für die Öffentlichkeit verfügbar ("Open Access") |
Lizenz (Deutsch): | Creative Commons - CC BY - Namensnennung 4.0 International |
Datum der Freischaltung: | 23.01.2018 |
Referierte Publikation: | Ja |
Datum der Eintragung als referierte Publikation: | 23.01.2018 |
Schriftenreihen ohne Nummerierung: | Wissenschaftliche Artikel der BAM |