Photophysics and release kinetics of enzyme-activatable optical probes based on H-dimerized fluorophores on self-immolative linkers
- A series of activatable optical probes for the model enzyme penicillin G amidase based on intramolecular formation of non-fluorescent H-dimer between two identical dyes were synthesized. The probes are based on a self-immolative linker, which allows positioning both dyes in close spatial proximity to ensure efficient quenching of probes with absorption and fluorescence emission in the near-infrared (NIR) range. A detailed photophysical investigation of the novel optical probes led to a revision of a previously anticipated quenching mechanism and revealed their potential for optimizing the performance of activatable probes based on H-dimer formation. A kinetic analysis indicated that the fluorescence progress curves can be used to qualitatively extract enzyme kinetic parameters.
MetadatenAutor*innen: | Jagoda Sloniec, Ute Resch-GengerORCiD, Andreas Hennig |
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Dokumenttyp: | Zeitschriftenartikel |
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Veröffentlichungsform: | Verlagsliteratur |
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Sprache: | Englisch |
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Titel des übergeordneten Werkes (Englisch): | The journal of physical chemistry / B |
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Jahr der Erstveröffentlichung: | 2013 |
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Herausgeber (Institution): | American Chemical Society |
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Verlag: | Soc. |
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Verlagsort: | Washington, DC |
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Jahrgang/Band: | 117 |
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Ausgabe/Heft: | 46 |
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Erste Seite: | 14336 |
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Letzte Seite: | 14344 |
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DOI: | 10.1021/jp409388b |
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ISSN: | 1520-6106 |
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ISSN: | 1520-5207 |
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ISSN: | 1089-5647 |
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Verfügbarkeit des Dokuments: | Datei im Netzwerk der BAM verfügbar ("Closed Access") |
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Datum der Freischaltung: | 20.02.2016 |
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Referierte Publikation: | Ja |
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Datum der Eintragung als referierte Publikation: | 27.01.2014 |
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